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Infection and Immunity, February 1999, p. 800-804, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Bacterial Invasion Is Not Required for Activation of NF-kappa B in Enterocytes

Tonyia Eaves-Pyles, Csaba Szabó, and Andrew L. Salzman*

Division of Critical Care, Children's Hospital Medical Center, Cincinnati, Ohio 45229

Received 1 June 1998/Returned for modification 29 July 1998/Accepted 19 November 1998

Pathogenic enteric microorganisms induce the NF-kappa B-dependent expression of proinflammatory genes in intestinal epithelial cells. The purpose of the present study was to clarify the contribution of microbial invasion to the degradation of the regulatory protein Ikappa Balpha and the subsequent activation of NF-kappa B in cultured intestinal epithelial cells. Caco-2BBe cells were incubated with Salmonella dublin, Salmonella typhimurium, or a weakly invasive strain of E. coli. S. dublin and S. typhimurium (107 organisms/ml) induced equivalent concentration-dependent gel mobility shifts of an NF-kappa B consensus sequence that was preceded by Ikappa Balpha degradation. E. coli (107 organisms/ml) did not induce Ikappa Balpha degradation or NF-kappa B translocation. Pretreatment with cytochalasin D blocked invasion of all three strains but had no effect on Ikappa Balpha degradation or NF-kappa B activation. S. dublin and S. typhimurium adhered to Caco-2BBe cells 3- to 10-fold more than E. coli. NF-kappa B activation was prevented by physical separation of S. dublin from Caco-2BBe cells by a 0.4-µm-pore-size filter. Our results imply that bacterial adhesion, rather than invasion or release of a secreted factor, is sufficient to induce Ikappa Balpha degradation and NF-kappa B activation in intestinal epithelial cells. Our data suggest that strategies to reduce enteric inflammation should be directed to the reduction of bacterial enterocyte adhesion.

* Corresponding author. Mailing address: Inotek Corporation, Third Floor, 3130 Highland Ave., Cincinnati, OH 45219-2374. Phone: (513) 475-6655. Fax: (513) 221-8079. E-mail: alsalzman{at}aol.com.

Infection and Immunity, February 1999, p. 800-804, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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