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Infection and Immunity, February 1999, p. 853-861, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Expression and Distribution of Leptospiral Outer Membrane Components during Renal Infection of Hamsters

Jeanne K. Barnett,1 Dean Barnett,1 Carole A. Bolin,2 Theresa A. Summers,3 Elizabeth A. Wagar,4 Norman F. Cheville,5 Rudy A. Hartskeerl,6 and David A. Haake3,7,*

Biology Department, University of Southern Indiana, Evansville, Indiana 477121; National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture,2 and Department of Pathology, College of Veterinary Medicine, Iowa State University,5 Ames, Iowa 50010; Division of Infectious Diseases, West Los Angeles Veterans Affairs Medical Center, Los Angeles, California 900733; Department of Pathology and Laboratory Medicine4 and Department of Medicine,7 UCLA School of Medicine, Los Angeles, California 90095; and Department of Biomedical Research, Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands6

Received 10 February 1998/Returned for modification 27 March 1998/Accepted 14 September 1998

The outer membrane of pathogenic Leptospira species grown in culture media contains lipopolysaccharide (LPS), a porin (OmpL1), and several lipoproteins, including LipL36 and LipL41. The purpose of this study was to characterize the expression and distribution of these outer membrane antigens during renal infection. Hamsters were challenged with host-derived Leptospira kirschneri to generate sera which contained antibodies to antigens expressed in vivo. Immunoblotting performed with sera from animals challenged with these host-derived organisms demonstrated reactivity with OmpL1, LipL41, and several other proteins but not with LipL36. Although LipL36 is a prominent outer membrane antigen of cultivated L. kirschneri, its expression also could not be detected in infected hamster kidney tissue by immunohistochemistry, indicating that expression of this protein is down-regulated in vivo. In contrast, LPS, OmpL1, and LipL41 were demonstrated on organisms colonizing the lumen of proximal convoluted renal tubules at both 10 and 28 days postinfection. Tubular epithelial cells around the luminal colonies had fine granular cytoplasmic LPS. When the cellular inflammatory response was present in the renal interstitium at 28 days postinfection, LPS and OmpL1 were also detectable within interstitial phagocytes. These data establish that outer membrane components expressed during infection have roles in the induction and persistence of leptospiral interstitial nephritis.

* Corresponding author. Mailing address: Division of Infectious Diseases, 111F, West Los Angeles Veterans Affairs Medical Center, Los Angeles, CA 90073. Phone: (310) 478-3711, ext. 40267. Fax: (310) 268-4928. E-mail: dhaake{at}ucla.edu.

Infection and Immunity, February 1999, p. 853-861, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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