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Infection and Immunity, February 1999, p. 946-953, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Human Intestinal M Cells Display the Sialyl Lewis A Antigen

Paul J. Giannasca,dagger Karen T. Giannasca, Alan M. Leichtner, and Marian R. Neutra*

Department of Pediatrics, Harvard Medical School, and Department of Medicine and GI Cell Biology Research Laboratory, Children's Hospital, Boston, Massachusetts 02115

Received 25 June 1998/Returned for modification 16 October 1998/Accepted 24 November 1998

The biochemical features that distinguish human M cells from other intestinal epithelial cell types are important for understanding microbial pathogenesis and for targeting vaccines to the mucosal immune system. We applied a large panel of carbohydrate-specific monoclonal antibodies and lectins to Peyer's patch and cecum biopsy specimens from three normal individuals and a patient with inflammatory bowel disease. The results show that human M-cell glycosylation patterns are distinct from those of other species examined and that human M cells preferentially display the sialyl Lewis A antigen. This carbohydrate epitope is also present in a small subpopulation of enterocytes in the follicle-associated epithelium and in goblet cell mucins.

* Corresponding author. Mailing address: GI Cell Biology, Enders 1220, Children's Hospital, 300 Longwood Ave., Boston, MA 02115. Phone: (617) 355-6229. Fax: (617) 730-0404. E-mail: neutra_m{at}a1.tch.harvard.edu.

dagger Present address: OraVax Inc., Cambridge, MA 02139.

Infection and Immunity, February 1999, p. 946-953, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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