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Infection and Immunity, February 1999, p. 986-988, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Vaccination with Live Escherichia coli Expressing Brucella abortus Cu/Zn Superoxide Dismutase Protects Mice against Virulent B. abortus

Angel A. Oñate,1,* Ramesh Vemulapalli,2 Edilia Andrews,1 Gerhardt G. Schurig,2 Stephen Boyle,2 and Hugo Folch3

Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, Concepción,1 and Department of Immunology, Faculty of Medicine, Universidad Austral, Valdivia,3 Chile, and Center for Molecular Medicine and Infectious Disease, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute & State University, Blacksburg, Virginia 240612

Received 13 April 1998/Returned for modification 18 August 1998/Accepted 20 October 1998

Vaccination of mice with Escherichia coli expressing Brucella Cu/Zn superoxide dismutase (SOD) [E. coli(pBSSOD)] induced a significant level of protection against virulent Brucella abortus challenge, although this level was not as high as the one reached with B. abortus vaccine strain RB51. In addition, vaccination with E. coli(pBSSOD) induced antibodies to Cu/Zn SOD and a strong proliferative response of splenocytes when stimulated in vitro with a thioredoxin-Cu/Zn SOD fusion protein.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepción, P.O. Box 152-C, Concepción, Chile. Phone: (056)-41-204118. Fax: (056)-41-245975. E-mail: aonate{at}udec.cl.

Infection and Immunity, February 1999, p. 986-988, Vol. 67, No. 2
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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