This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Morikawa, A.
Right arrow Articles by Yokochi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Morikawa, A.
Right arrow Articles by Yokochi, T.

 Previous Article  |  Next Article 

Infection and Immunity, March 1999, p. 1018-1024, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Role of Nitric Oxide in Lipopolysaccharide-Induced Hepatic Injury in D-Galactosamine-Sensitized Mice as an Experimental Endotoxic Shock Model

Akiko Morikawa, Yutaka Kato, Tuyoshi Sugiyama, Naoki Koide, Dipshika Chakravortty, Tomoaki Yoshida, and Takashi Yokochi*

Department of Microbiology and Immunology and Division of Bacterial Toxin, Research Center for Infectious Disease, Aichi Medical University, Nagakute, Aichi 480-1195, Japan

Received 29 July 1998/Returned for modification 15 October 1998/Accepted 4 December 1998

The role of nitric oxide (NO) in lipopolysaccharide (LPS)-induced hepatic injury was studied in D-galactosamine (D-GalN)-sensitized mice. The inducible isoform of NO synthase (iNOS) was immunohistochemically detected on hepatocytes around blood vessels in livers of mice injected with D-GalN and LPS not on hepatocytes in mice injected with D-GalN or LPS alone, although mRNA for iNOS was found in those mice. Nitrotyrosine (NT) was also found in livers of mice injected with D-GalN and LPS. The localization of NT was consistent with that of iNOS, and the time courses of NT and iNOS expression were almost the same. Expression of iNOS and NT was detected exclusively in the hepatic lesions of mice injected with D-GalN and LPS. Anti-tumor necrosis factor alpha neutralizing antibody inhibited iNOS and NT expression and hepatic injury. The results suggested that NO from iNOS may play a role in LPS-induced hepatic injury on D-GalN-sensitized mice as an experimental endotoxic shock model.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Aichi Medical University, Nagakute, Aichi 480-1195, Japan. Phone: 81-561-62-3311, ext. 2269. Fax: 81-561-63-9187. E-mail: yokochi{at}amugw.aichi-med-u.ac.jp.

Infection and Immunity, March 1999, p. 1018-1024, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Eswarappa, S. M., Pareek, V., Chakravortty, D. (2008). Role of actin cytoskeleton in LPS-induced NF-{kappa}B activation and nitric oxide production in murine macrophages. Innate Immunity 14: 309-318 [Abstract]  
  • Zeini, M., Traves, P. G., Lopez-Fontal, R., Pantoja, C., Matheu, A., Serrano, M., Bosca, L., Hortelano, S. (2006). Specific Contribution of p19ARF to Nitric Oxide-Dependent Apoptosis.. J. Immunol. 177: 3327-3336 [Abstract] [Full Text]  
  • Ito, H., Koide, N., Morikawa, A., Hassan, F., Islam, S., Tumurkhuu, G., Mori, I., Yoshida, T., Kakumu, S., Moriwaki, H., Yokochi, T. (2005). Augmentation of lipopolysaccharide-induced nitric oxide production by {alpha}-galactosylceramide in mouse peritoneal cells. Innate Immunity 11: 213-219 [Abstract]  
  • Huang, H., Rose, J. L., Hoyt, D. G. (2004). p38 Mitogen-Activated Protein Kinase Mediates Synergistic Induction of Inducible Nitric-Oxide Synthase by Lipopolysaccharide and Interferon-{gamma} through Signal Transducer and Activator of Transcription 1 Ser727 Phosphorylation in Murine Aortic Endothelial Cells. Mol. Pharmacol. 66: 302-311 [Abstract] [Full Text]  
  • Sugiyama, T., Fujita, M., Koide, N., Morikawa, A., Takahashi, K., Yoshida, T., Mori, H., Yokochi, T. (2003). Differences in the mechanism of nitric oxide production between mouse vascular endothelial cells and macrophages. Innate Immunity 9: 108-112 [Abstract]  
  • Halpern, M. D., Holubec, H., Dominguez, J. A., Meza, Y. G., Williams, C. S., Ruth, M. C., McCuskey, R. S., Dvorak, B. (2003). Hepatic inflammatory mediators contribute to intestinal damage in necrotizing enterocolitis. Am. J. Physiol. Gastrointest. Liver Physiol. 284: G695-G702 [Abstract] [Full Text]  
  • Soderberg, M., Raffalli-Mathieu, F., Lang, M. A. (2002). Inflammation Modulates the Interaction of Heterogeneous Nuclear Ribonucleoprotein (hnRNP) I/Polypyrimidine Tract Binding Protein and hnRNP L with the 3'Untranslated Region of the Murine Inducible Nitric-Oxide Synthase mRNA. Mol. Pharmacol. 62: 423-431 [Abstract] [Full Text]  
  • Mya Mya Mu, , Chakravortty, D., Sugiyama, T., Koide, N., Takahashi, K., Mori, I., Yoshida, T., Yokochi, T. (2001). The inhibitory action of quercetin on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells. Innate Immunity 7: 431-438 [Abstract]  
  • Nadler, E. P., Dickinson, E. C., Beer-Stolz, D., Alber, S. M., Watkins, S. C., Pratt, D. W., Ford, H. R. (2001). Scavenging nitric oxide reduces hepatocellular injury after endotoxin challenge. Am. J. Physiol. Gastrointest. Liver Physiol. 281: G173-G181 [Abstract] [Full Text]  
  • Sugiyama, T., Koide, N., Chakravortty, D., Kato, Y., Mu, M. M., Yoshida, T., Yokochi, T. (2001). The expression of membrane-bound CD14 renders mouse B-1 cells susceptible to LPS. Innate Immunity 7: 223-226 [Abstract]  
  • Chakravortty, D., Kato, Y., Sugiyama, T., Koide, N., Mu, M. M., Yoshida, T., Yokochi, T. (2001). Inhibition of Caspase 3 Abrogates Lipopolysaccharide-Induced Nitric Oxide Production by Preventing Activation of NF-{kappa}B and c-Jun NH2-Terminal Kinase/Stress-Activated Protein Kinase in RAW 264.7 Murine Macrophage Cells. Infect. Immun. 69: 1315-1321 [Abstract] [Full Text]  
  • Koide, N., Sugiyama, T., Kato, Y., Chakravortty, D., Mya Mya Mu, , Yoshida, T., Hamano, T., Yokochi, T. (2001). Mouse B1 cell line responds to lipopolysaccharide via membrane-bound CD14. Innate Immunity 7: 39-43 [Abstract]  
  • Chakravortty, D., Kato, Y., Sugiyama, T., Koide, N., Mu, M. M., Yoshida, T., Yokochi, T. (2001). The Inhibitory Action of Sodium Arsenite on Lipopolysaccharide-Induced Nitric Oxide Production in RAW 267.4 Macrophage Cells: A Role of Raf-1 in Lipopolysaccharide Signaling. J. Immunol. 166: 2011-2017 [Abstract] [Full Text]  
  • Morikawa, A., Koide, N., Kato, Y., Sugiyama, T., Chakravortty, D., Yoshida, T., Yokochi, T. (2000). Augmentation of Nitric Oxide Production by Gamma Interferon in a Mouse Vascular Endothelial Cell Line and Its Modulation by Tumor Necrosis Factor Alpha and Lipopolysaccharide. Infect. Immun. 68: 6209-6214 [Abstract] [Full Text]  
  • Ohta, A., Sekimoto, M., Sato, M., Koda, T., Nishimura, S.-i., Iwakura, Y., Sekikawa, K., Nishimura, T. (2000). Indispensable Role for TNF-{alpha} and IFN-{gamma} at the Effector Phase of Liver Injury Mediated by Th1 Cells Specific to Hepatitis B Virus Surface Antigen. J. Immunol. 165: 956-961 [Abstract] [Full Text]  
  • Wang, F., Wang, L. Y., Wright, D., Parmely, M. J. (1999). Redox Imbalance Differentially Inhibits Lipopolysaccharide-Induced Macrophage Activation in the Mouse Liver. Infect. Immun. 67: 5409-5416 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»