This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sun, Y.
Right arrow Articles by Nahm, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sun, Y.
Right arrow Articles by Nahm, M. H.

 Previous Article  |  Next Article 

Infection and Immunity, March 1999, p. 1172-1179, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Repertoire of Human Antibodies against the Polysaccharide Capsule of Streptococcus pneumoniae Serotype 6B

Yan Sun,1 Moon K. Park,2 Jihye Kim,2 Betty Diamond,3 Alan Solomon,4 and Moon H. Nahm1,*

Departments of Pediatrics, Pathology, and Medicine, University of Rochester, Rochester, New York 146421; Faculty of Biological Sciences, Chonbuk National University Chonju, 561-756, Korea2; Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 104613; and Human Immunology and Cancer Program, Department of Medicine, University of Tennessee Medical Center/Graduate School of Medicine, Knoxville, Tennessee 379204

Received 12 June 1998/Returned for modification 5 August 1998/Accepted 1 October 1998

We examined the repertoire of antibodies to Streptococcus pneumoniae 6B capsular polysaccharide induced with the conventional polysaccharide vaccine in adults at the molecular level two ways. In the first, we purified from the sera of seven vaccinees antipneumococcal antibodies and determined their amino acid sequences. Their VH regions are mainly the products of VH3 family genes (candidate genes, 3-23, 3-07, 3-66, and 3-74), but the product of a VH1 family gene (candidate gene, 1-03) is occasionally used. All seven individuals have small amounts of polyclonal kappa + antibodies (Vkappa 1 to Vkappa 4 families), although kappa + antibodies are occasionally dominated by antibodies formed with the product of the A27 Vkappa gene. In contrast, lambda + anti-6B antibodies are dominated by the antibodies derived from one of 3 very similar Vlambda 2 family genes (candidate genes, 2c, 2e, and 2a2) and Clambda 1 gene product. The Vlambda 2+ antibodies express the 8.12 idiotype, which is expressed on anti-double-stranded-DNA antibodies. In one case, Vlambda is derived from a rarely expressed Vlambda gene, 10a. In the second approach, we studied a human hybridoma (Dob1) producing anti-6B antibody. Its VH region sequence is closely related to those of the 3-15 VH gene (88% nucleotide homology) and JH4 (92% homology). Its VL region is homologous to the 2a2 Vlambda 2 gene (91%) and Jlambda 1/Clambda 1. Taken together, the V region of human anti-6B antibodies is commonly formed by a VH3 and a Vlambda 2 family gene product.

* Corresponding author. Mailing address: Departments of Pediatrics, Pathology, and Medicine, University of Rochester, 601 Elmwood Ave., Box 777, Rochester, NY 14642-8777. Phone: (716) 275-7963. Fax: (716) 271-7512. E-mail: moon{at}vaccine.rochester.edu.

Infection and Immunity, March 1999, p. 1172-1179, Vol. 67, No. 3
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Park, S., Parameswar, A. R., Demchenko, A. V., Nahm, M. H. (2009). Identification of a Simple Chemical Structure Associated with Protective Human Antibodies against Multiple Pneumococcal Serogroups. Infect. Immun. 77: 3374-3379 [Abstract] [Full Text]  
  • Baxendale, H. E., Goldblatt, D. (2006). Correlation of Molecular Characteristics, Isotype, and In Vitro Functional Activity of Human Antipneumococcal Monoclonal Antibodies. Infect. Immun. 74: 1025-1031 [Abstract] [Full Text]  
  • Kolibab, K., Smithson, S. L., Rabquer, B., Khuder, S., Westerink, M. A. J. (2005). Immune Response to Pneumococcal Polysaccharides 4 and 14 in Elderly and Young Adults: Analysis of the Variable Heavy Chain Repertoire. Infect. Immun. 73: 7465-7476 [Abstract] [Full Text]  
  • Yu, J., Lin, J., Benjamin, W. H. Jr., Waites, K. B., Lee, C.-h., Nahm, M. H. (2005). Rapid Multiplex Assay for Serotyping Pneumococci with Monoclonal and Polyclonal Antibodies. J. Clin. Microbiol. 43: 156-162 [Abstract] [Full Text]  
  • Zhou, J., Lottenbach, K. R., Barenkamp, S. J., Reason, D. C. (2004). Somatic Hypermutation and Diverse Immunoglobulin Gene Usage in the Human Antibody Response to the Capsular Polysaccharide of Streptococcus pneumoniae Type 6B. Infect. Immun. 72: 3505-3514 [Abstract] [Full Text]  
  • Weitkamp, J.-H., Kallewaard, N., Kusuhara, K., Bures, E., Williams, J. V., LaFleur, B., Greenberg, H. B., Crowe, J. E. Jr. (2003). Infant and Adult Human B Cell Responses to Rotavirus Share Common Immunodominant Variable Gene Repertoires. J. Immunol. 171: 4680-4688 [Abstract] [Full Text]  
  • Tachibana, H., Watanabe, K., Cheng, X.-J., Tsukamoto, H., Kaneda, Y., Takeuchi, T., Ihara, S., Petri, W. A. Jr. (2003). VH3 Gene Usage in Neutralizing Human Antibodies Specific for the Entamoeba histolytica Gal/GalNAc Lectin Heavy Subunit. Infect. Immun. 71: 4313-4319 [Abstract] [Full Text]  
  • Saeland, E., Vidarsson, G., Leusen, J. H. W., van Garderen, E., Nahm, M. H., Vile-Weekhout, H., Walraven, V., Stemerding, A. M., Verbeek, J. S., Rijkers, G. T., Kuis, W., Sanders, E. A. M., van de Winkel, J. G. J. (2003). Central Role of Complement in Passive Protection by Human IgG1 and IgG2 Anti-pneumococcal Antibodies in Mice. J. Immunol. 170: 6158-6164 [Abstract] [Full Text]  
  • Chang, Q., Zhong, Z., Lees, A., Pekna, M., Pirofski, L. (2002). Structure-Function Relationships for Human Antibodies to Pneumococcal Capsular Polysaccharide from Transgenic Mice with Human Immunoglobulin Loci. Infect. Immun. 70: 4977-4986 [Abstract] [Full Text]  
  • Zhou, J., Lottenbach, K. R., Barenkamp, S. J., Lucas, A. H., Reason, D. C. (2002). Recurrent Variable Region Gene Usage and Somatic Mutation in the Human Antibody Response to the Capsular Polysaccharide of Streptococcus pneumoniae Type 23F. Infect. Immun. 70: 4083-4091 [Abstract] [Full Text]  
  • Shin, J.-S., Yu, J., Lin, J., Zhong, L., Bren, K. L., Nahm2, M. H. (2002). Peptide Mimotopes of Pneumococcal Capsular Polysaccharide of 6B Serotype: A Peptide Mimotope Can Bind to Two Unrelated Antibodies. J. Immunol. 168: 6273-6278 [Abstract] [Full Text]  
  • Hemachandra, S., Kamboj, K., Copfer, J., Pier, G., Green, L. L., Schreiber, J. R. (2001). Human Monoclonal Antibodies against Pseudomonas aeruginosa Lipopolysaccharide Derived from Transgenic Mice Containing Megabase Human Immunoglobulin Loci Are Opsonic and Protective against Fatal Pseudomonas Sepsis. Infect. Immun. 69: 2223-2229 [Abstract] [Full Text]  
  • Lucas, A. H., Moulton, K. D., Tang, V. R., Reason, D. C. (2001). Combinatorial Library Cloning of Human Antibodies to Streptococcus pneumoniae Capsular Polysaccharides: Variable Region Primary Structures and Evidence for Somatic Mutation of Fab Fragments Specific for Capsular Serotypes 6B, 14, and 23F. Infect. Immun. 69: 853-864 [Abstract] [Full Text]  
  • Scamurra, R. W., Miller, D. J., Dahl, L., Abrahamsen, M., Kapur, V., Wahl, S. M., Milner, E. C. B., Janoff, E. N. (2000). Impact of HIV-1 Infection on VH3 Gene Repertoire of Naive Human B Cells. J. Immunol. 164: 5482-5491 [Abstract] [Full Text]  
  • Park, M. K., Briles, D. E., Nahm, M. H. (2000). A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay). CVI 7: 486-489 [Abstract] [Full Text]  
  • Russell, N. D., Corvalan, J. R. F., Gallo, M. L., Davis, C. G., Pirofski, L.-a. (2000). Production of Protective Human Antipneumococcal Antibodies by Transgenic Mice with Human Immunoglobulin Loci. Infect. Immun. 68: 1820-1826 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»