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Infection and Immunity, March 1999, p. 1292-1296, Vol. 67, No. 3
0019-9567/99

Enhanced Adhesion of Pasteurella multocida to Cultured Turkey Peripheral Blood Monocytes

Ingrid M. Pruimboom,dagger Richard B. Rimler,* and Mark R. Ackermanndagger

Avian and Swine Respiratory Diseases Research Unit, USDA Agricultural Research Service National Animal Disease Center, Ames, Iowa 50010

Received 22 December 1997/Returned for modification 12 March 1998/Accepted 3 December 1998

Capsular hyaluronic acid (HA) mediates adhesion of serogroup A strains of Pasteurella multocida to elicited turkey air sac macrophages (TASM). In contrast, freshly isolated turkey peripheral blood monocytes (TPBM) do not bind serogroup A strains. Following culture of TPBM for 6 days in chamber slides, adhesion of the bacteria to TPBM increased gradually. Incubation in chamber slides coated with entactin-collagen IV-laminin (ECL) attachment matrix or exposure to phorbol myristate acetate (PMA) further enhanced the adhesion of P. multocida to TPBM. Addition of HA, but not Arg-Gly-Asp peptide, to TPBM culture inhibited bacterial adherence similarly to the inhibition previously reported for TASM. Exposure of TPBM to monoclonal antibody directed against HA-binding cell surface proteoglycan (CD44) decreased binding of P. multocida. Collectively, these findings indicate that P. multocida adhesion to TPBM is mediated by capsular HA and can be increased by culture on ECL attachment matrix or PMA exposure. Additionally, the findings suggest that the capsular mucopolysaccharide of serogroup A strains of P. multocida recognizes an isoform of CD44 expressed on cultured TPBM.

* Corresponding author. Mailing address: USDA/ARS/National Animal Disease Center, P.O. Box 70, Ames, IA 50010. Phone: (515) 239-8255. Fax: (515) 239-8458. E-mail: rrimler{at}nadc.ars.usda.gov.

dagger Present address: Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, IA 50011.

Infection and Immunity, March 1999, p. 1292-1296, Vol. 67, No. 3
0019-9567/99


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