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 Previous Article

Infection and Immunity, March 1999, p. 1533-1537, Vol. 67, No. 3
0019-9567/99

Protective Efficacy of Recombinant Yersinia Outer Proteins against Bubonic Plague Caused by Encapsulated and Nonencapsulated Yersinia pestis

G. P. Andrews,* S. T. Strachan, G. E. Benner, A. K. Sample, G. W. Anderson Jr.,dagger J. J. Adamovicz, S. L. Welkos, J. K. Pullen, and A. M. Friedlander*

Division of Bacteriology, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011

Received 8 September 1998/Returned for modification 5 October 1998/Accepted 21 December 1998

To evaluate the role of Yersinia outer proteins (Yops) in conferring protective immunity against plague, six yop loci from Yersinia pestis were individually amplified by PCR, cloned, and expressed in Escherichia coli. The recombinant proteins were purified and injected into mice. Most Yop-vaccinated animals succumbed to infection with either wild-type encapsulated Y. pestis or a virulent, nonencapsulated isogenic variant. Vaccination with YpkA significantly prolonged mean survival time but did not increase overall survival of mice infected with the nonencapsulated strain. The only significant protection against death was observed in YopD-vaccinated mice challenged with the nonencapsulated strain.

* Corresponding author. Mailing address for G. P. Andrews: Department of Pathology & Area Laboratory Services, Landstuhl Regional Medical Center, D-66849 Landstuhl, Germany. Mailing address for A. M. Friedlander: Division of Bacteriology, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011. Phone: (301)-619-7341. Fax: (301)-619-2152. E-mail: friedlan{at}ncifcrf.gov.

dagger Present address: Southern Research Institute, Frederick, MD 21701-4756.

Infection and Immunity, March 1999, p. 1533-1537, Vol. 67, No. 3
0019-9567/99


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