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Infection and Immunity, April 1999, p. 1677-1682, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Transformed Toxoplasma gondii Tachyzoites Expressing the Circumsporozoite Protein of Plasmodium knowlesi Elicit a Specific Immune Response in Rhesus Monkeys

Manlio di Cristina,1 Firman Ghouze,1 Clemens H. M. Kocken,2 Silvia Naitza,1 Pamela Cellini,3 Dominique Soldati,4 Alan W. Thomas,2 and Andrea Crisanti1,*

Department of Biology, Imperial College, SW7 2BB London, United Kingdom1; Department of Parasitology, Biomedical Primate Research Centre, 2288 GJ Rijwijk, The Netherlands2; Istituto di Parassitologia, Università di Roma "La Sapienza," 00185 Rome, Italy3; and Zentrum für Molekulare Biologie, Universität Heidelberg, Heidelberg 6900, Germany4

Received 13 July 1998/Returned for modification 23 September 1998/Accepted 8 December 1998

Toxoplasma gondii tachyzoites were transformed with the coding sequence of the circumsporozoite (CS) protein of the primate malaria parasite Plasmodium knowlesi. A single inoculation of live transformed tachyzoites elicited an antibody response directed against the immunodominant repeat epitope (EQPAAGAGG)2 of the P. knowlesi CS protein in rhesus monkeys. Notably, these animals failed to show a positive serum conversion against T. gondii. Antibodies against Toxoplasma antigens were detected only after a second inoculation with a higher number of transformed tachyzoites. This boost induced an increased antibody response against the P. knowlesi CS protein associated with immunoglobulin class switching, thus demonstrating the establishment of immunological memory. These results indicate that the Toxoplasma-derived CS protein is efficiently recognized by the monkey immune system and represents an immunodominant antigen in transformed parasites.

* Corresponding author. Mailing address: Imperial College, Department of Biology, Prince Consort Rd., SW7 2BB London, United Kingdom. Phone: 44 171 5945426. Fax: 44 171 5945439. E-mail: acrs{at}bio.ic.ac.uk.

Infection and Immunity, April 1999, p. 1677-1682, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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