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Infection and Immunity, April 1999, p. 1821-1827, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antibodies Reactive with the N-Terminal Domain of Plasmodium falciparum Serine Repeat Antigen Inhibit Cell Proliferation by Agglutinating Merozoites and Schizonts

Xin-Li Pang, Toshihide Mitamura, and Toshihiro Horii*

Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, Suita Osaka 565-0871, Japan

Received 29 September 1998/Returned for modification 29 October 1998/Accepted 5 January 1999

The serine repeat antigen (SERA) is a vaccine candidate antigen of Plasmodium falciparum. Immunization of mice with Escherichia coli-produced recombinant protein of the SERA N-terminal domain (SE47') induced an antiserum that was inhibitory to parasite growth in vitro. Affinity-purified mouse antibodies specific to the recombinant protein inhibited parasite growth between the schizont and ring stages but not between the ring and schizont stages. When Percoll-purified schizonts were cultured with the affinity-purified SE47'-specific antibodies, schizonts and merozoites were agglutinated. Indirect-immunofluorescence assays with unfixed parasite cells showed that SE47'-specific immunoglobulin G (IgG) bound to SERA molecules on rupturing schizonts and merozoites but the IgG did not react with the schizont-infected erythrocytes (RBC). Furthermore, double-fluorescence staining against SE47'-specific IgG and anti-human RBC membrane IgG showed that the RBC membrane disappeared from SE47'-specific-IgG-bound schizonts after cultivation. These observations suggest that the SE47'-specific antibodies inhibit parasite growth by cross-linking SERA molecules that are associated with merozoites in rupturing schizonts with partly broken RBC and parasitophorous vacuole membranes, blocking merozoite release.

* Corresponding author. Mailing address: Department of Molecular Protozoology, Research Institute for Microbial Diseases, Osaka University, Suita Osaka 565-0781, Japan. Phone: 81-6-6879-8280. Fax: 81- 6-6879-8281. E-mail: horii{at}biken.osaka-u.ac.jp.

Infection and Immunity, April 1999, p. 1821-1827, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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