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Infection and Immunity, April 1999, p. 1910-1916, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Characterization of a Novel Trypanosome Lytic Factor from Human Serum

Jayne Raper,1,* Ramie Fung,1 Jorge Ghiso,2 Victor Nussenzweig,2 and Stephen Tomlinson2

Departments of Medical and Molecular Parasitology1 and Pathology,2 New York University Medical School, New York, New York 10010

Received 27 May 1998/Returned for modification 27 July 1998/Accepted 2 December 1998

Natural resistance of humans to the cattle pathogen Trypanosoma brucei brucei has been attributed to the presence in human serum of nonimmune factors that lyse the parasite. Normal human serum contains two trypanosome lytic factors (TLFs). TLF1 is a 500-kDa lipoprotein, which is reported to contain apolipoprotein A-I (apoA-I), haptoglobin-related protein (Hpr), hemoglobin, paraoxonase, and apoA-II, whereas TLF2 is a larger, poorly characterized particle. We report here a new immunoaffinity-based purification procedure for TLF2 and TLF1, as well as further characterization of the components of each purified TLF. Immunoaffinity-purified TLF1 has a specific activity 10-fold higher than that of TLF1 purified by previously described methods. Moreover, we find that TLF1 is a lipoprotein particle that contains mainly apoA-I and Hpr, trace amounts of paraoxonase, apoA-II, and haptoglobin, but no detectable hemoglobin. Characterization of TLF2 reveals that it is a 1,000-kDa protein complex containing mainly immunoglobulin M, apoA-I, and Hpr but less than 1% detectable lipid.

* Corresponding author. Mailing address: Department of Medical and Molecular Parasitology, New York University School of Medicine, 341 East 25th St., New York, NY 10010. Phone: (212) 263-7632. Fax: (212) 263-8179. E-mail: raperj01{at}med.nyu.edu.

Infection and Immunity, April 1999, p. 1910-1916, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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