This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Piacenza, L.
Right arrow Articles by Carmona, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Piacenza, L.
Right arrow Articles by Carmona, C.

 Previous Article  |  Next Article 

Infection and Immunity, April 1999, p. 1954-1961, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Vaccination with Cathepsin L Proteinases and with Leucine Aminopeptidase Induces High Levels of Protection against Fascioliasis in Sheep

Lucía Piacenza,1 Daniel Acosta,1 Isabel Basmadjian,1 John P. Dalton,2 and Carlos Carmona1,*

Unidad de Biología Parasitaria, Facultad de Ciencias, Instituto de Higiene, Montevideo, Uruguay,1 and School of Biological Sciences, Dublin City University, Glasnevin, Dublin 9, Republic of Ireland2

Received 13 July 1998/Returned for modification 4 September 1998/Accepted 26 January 1999

The potential of different parasite proteinases for use as vaccine candidates against fascioliasis in sheep was studied by vaccinating animals with the cathepsin L proteinases CL1 and CL2 and with leucine aminopeptidase (LAP) purified from adult flukes. In the first trial, sheep were immunized with CL1 or CL2 and the mean protection levels obtained were 33 and 34%, respectively. Furthermore, a significant reduction in egg output was observed in sheep vaccinated either with CL1 (71%) or with CL2 (81%). The second trial was performed to determine the protective potential of the two cathepsin L proteinases assayed together, as well as in combination with LAP, and of LAP alone. The combination of CL1 and CL2 induced higher levels of protection (60%) than those produced when these enzymes were administered separately. Those sheep that received the cocktail vaccine including CL1, CL2, and LAP were significantly protected (78%) against metacercarial challenge, but vaccination with LAP alone elicited the highest level of protection (89%). All vaccine preparations induced high immunoglobulin G titers which were boosted after the challenge infection, but no correlations between antibody titers and worm burdens were found. However, the sera of those animals vaccinated with LAP contained LAP-neutralizing antibodies. Reduced liver damage, as assessed by the level of the liver enzyme gamma-glutamyl transferase, was observed in the groups vaccinated with CL1, CL2, and LAP or with LAP alone.

* Corresponding author. Mailing address: Unidad de Biología Parasitaria, Faculdad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay. Phone: (598-2)-4801597. Fax: (598-2)-4873073. E-mail: ccarmona{at}higiene.edu.uy.

Infection and Immunity, April 1999, p. 1954-1961, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pearson, M. S., Bethony, J. M., Pickering, D. A., de Oliveira, L. M., Jariwala, A., Santiago, H., Miles, A. P., Zhan, B., Jiang, D., Ranjit, N., Mulvenna, J., Tribolet, L., Plieskatt, J., Smith, T., Bottazzi, M. E., Jones, K., Keegan, B., Hotez, P. J., Loukas, A. (2009). An enzymatically inactivated hemoglobinase from Necator americanus induces neutralizing antibodies against multiple hookworm species and protects dogs against heterologous hookworm infection. FASEB J. 23: 3007-3019 [Abstract] [Full Text]  
  • Marcilla, A., De la Rubia, J. E., Sotillo, J., Bernal, D., Carmona, C., Villavicencio, Z., Acosta, D., Tort, J., Bornay, F. J., Esteban, J. G., Toledo, R. (2008). Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections. CVI 15: 95-100 [Abstract] [Full Text]  
  • Bertin, P. B., Lozzi, S. P., Howell, J. K., Restrepo-Cadavid, G., Neves, D., Teixeira, A. R. L., de Sousa, M. V., Norris, S. J., Santana, J. M. (2005). The Thermophilic, Homohexameric Aminopeptidase of Borrelia burgdorferi Is a Member of the M29 Family of Metallopeptidases. Infect. Immun. 73: 2253-2261 [Abstract] [Full Text]  
  • Kedzierski, L., Black, C. G., Goschnick, M. W., Stowers, A. W., Coppel, R. L. (2002). Immunization with a Combination of Merozoite Surface Proteins 4/5 and 1 Enhances Protection against Lethal Challenge with Plasmodium yoelii. Infect. Immun. 70: 6606-6613 [Abstract] [Full Text]  
  • Morty, R. E., Morehead, J. (2002). Cloning and Characterization of a Leucyl Aminopeptidase from Three Pathogenic Leishmania Species. J. Biol. Chem. 277: 26057-26065 [Abstract] [Full Text]  
  • Yun, D.-H., Chung, J.-Y., Chung, Y.-B., Bahk, Y.-Y., Kang, S.-Y., Kong, Y., Cho, S.-Y. (2000). Structural and Immunological Characteristics of a 28-Kilodalton Cruzipain-Like Cysteine Protease of Paragonimus westermani Expressed in the Definitive Host Stage. CVI 7: 932-939 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»