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Infection and Immunity, April 1999, p. 2040-2044, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Biological Effects of Pseudomonas aeruginosa Type III-Secreted Proteins on CHO Cells

Amy J. Vallis, Viviane Finck-Barbançon, Timothy L. Yahr, and Dara W. Frank*

Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226

Received 9 December 1998/Accepted 6 January 1999

A strain of Pseudomonas aeruginosa that fails to express known type III-secreted effector proteins was constructed as an expression host. Individual effectors were expressed in trans, and their biological effects on CHO cells were assessed in an acute cellular infection model. Intoxication with ExoS, ExoT, or ExoY resulted in alterations in cell morphology. As shown in previous genetic studies, ExoU expression was linked to acute cytotoxicity.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226. Phone: (414) 456-8766. Fax: (414) 456-6535. E-mail: frankd{at}post.its.mcw.edu.

Infection and Immunity, April 1999, p. 2040-2044, Vol. 67, No. 4
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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