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Infection and Immunity, May 1999, p. 2218-2224, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antibody Response to Cryptococcus neoformans Proteins in Rodents and Humans

Lin-Chi Chen,1 David L. Goldman,2 Tamara L. Doering,3 Liise-anne Pirofski,1,4 and Arturo Casadevall1,4,*

Department of Microbiology and Immunology,1 Department of Pediatrics,2 and Division of Infectious Diseases, Department of Medicine,4 Albert Einstein College of Medicine, Bronx, New York 10461-2187, and Department of Pharmacology, Cornell University Medical College, New York, New York 10461-21873

Received 22 December 1998/Returned for modification 29 January 1999/Accepted 11 February 1999

The prevalence and specificity of serum antibodies to Cryptococcus neoformans proteins was studied in mice and rats with experimental infection, in individuals with or without a history of potential laboratory exposure to C. neoformans, human immunodeficiency virus (HIV)-positive individuals who developed cryptococcosis, in matched samples from HIV-positive individuals who did not develop cryptococcosis, and in HIV-negative individuals. Rodents had little or no serum antibody reactive with C. neoformans proteins prior to infection. The intensity and specificity of the rodent antibody response were dependent on the species, the mouse strain, and the viability of the inoculum. All humans had serum antibodies reactive with C. neoformans proteins regardless of the potential exposure, the HIV infection status, or the subsequent development of cryptococcosis. Our results indicate (i) a high prevalence of antibodies reactive with C. neoformans proteins in the sera of rodents after cryptococcal infection and in humans with or without HIV infection; (ii) qualitative and quantitative differences in the antibody profiles of HIV-positive individuals; and (iii) similarities and differences between humans, mice, and rats with respect to the specificity of the antibodies reactive with C. neoformans proteins. The results are consistent with the view that C. neoformans infections are common in human populations, and the results have implications for the development of vaccination strategies against cryptococcosis.

* Corresponding author. Mailing address: Albert Einstein College of Medicine, 701 Golding, 1300 Morris Park Ave., Bronx, NY 10461-2187. Phone: (718) 430-4259. Fax: (718) 430-8701. E-mail: casadeva{at}aecom.yu.edu.

Infection and Immunity, May 1999, p. 2218-2224, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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