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Infection and Immunity, May 1999, p. 2399-2405, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Molecular Interactions of Porphyromonas gingivalis Fimbriae with Host Proteins: Kinetic Analyses Based on Surface Plasmon Resonance

Atsuo Amano,1,* Takayuki Nakamura,2 Shigenobu Kimura,2 Ichijiro Morisaki,1 Ichiro Nakagawa,2 Shigetada Kawabata,2 and Shigeyuki Hamada2

Division of Special Care Dentistry,1 and Department of Oral Microbiology,2 Osaka University Faculty of Dentistry, Suita-Osaka, Japan

Received 2 November 1998/Returned for modification 18 December 1998/Accepted 23 February 1999

Fimbriae of Porphyromonas gingivalis are thought to play an important role in the colonization and invasion of periodontal tissues. In this study, we analyzed the interactions of P. gingivalis fimbriae with human hemoglobin, fibrinogen, and salivary components (i.e., proline-rich protein [PRP], proline-rich glycoprotein [PRG], and statherin) based on surface plasmon resonance (SPR) spectroscopy with a biomolecular interaction analyzing system (BIAcore). The real-time observation showed that the fimbriae interacted more quickly with hemoglobin and PRG than with other proteins and more intensely with fibrinogen. The significant association constant (ka) values obtained by BIAcore demonstrated that the interactions between fimbriae and these host proteins are specific. These estimated Ka values were not too different; however, the Ka values for hemoglobin (2.43 × 106) and fibrinogen (2.16 × 106) were statistically greater than those for the salivary proteins (1.48 × 106 to 1.63 × 106). The Ka value of anti-fimbriae immunoglobulin G for fimbriae was estimated to be 1.22 × 107, which was 6.55-fold higher than the mean Ka value of the host proteins. Peptide PRP-C, a potent inhibitor of PRP-fimbriae interaction, dramatically inhibited fimbrial association to PRP and PRG and was also inhibitory against other host proteins by BIAcore. The binding of fimbriae to these proteins was also evaluated by other methods with hydroxyapatite beads or polystyrene microtiter plates. The estimated binding abilities differed considerably, depending on the assay method that was used. It was noted that the binding capacity of PRP was strongly diminished by immobilization on a polystyrene surface. Taken together, these findings suggest that P. gingivalis fimbriae possess a strong ability to interact with the host proteins which promote bacterial adherence to the oral cavity and that SPR spectroscopy is a useful method for analyzing specific protein-fimbriae interactions.

* Corresponding author. Mailing address: Division of Special Care Dentistry, Osaka University Faculty of Dentistry, 1-8 Yamadaoka, Suita-Osaka 565-0871, Japan. Phone: 81-6-6879-2280. Fax: 81-6-6879-2284. E-mail: amanoa{at}dent.osaka-u.ac.jp.

Infection and Immunity, May 1999, p. 2399-2405, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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