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Infection and Immunity, May 1999, p. 2638-2642, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antibody to Glucosyltransferase Induced by Synthetic Peptides Associated with Catalytic Regions of -Amylases

Daniel J. Smith,^* Rhonda L. Heschel, William F. King, and Martin A. Taubman

Department of Immunology, Forsyth Dental Center, Boston, Massachusetts 02115

Received 3 December 1998/Returned for modification 8 January 1999/Accepted 3 February 1999

We examined the immunogenicity and induction of inhibitory activity of 19-mer synthetic peptides which contained putative catalytic regions that were associated with the ₅ (EAW) and ₇ (HDS) strand elements of the suggested (,)₈ catalytic barrel domain of Streptococcus mutans glucosyltransferase (GTF). Both peptides readily induced serum immunoglobulin G (IgG) and salivary IgA antipeptide activity which was reactive both with the inciting peptide and with intact S. mutans GTF. Antisera to each peptide construct also inhibited the ability of S. mutans GTF to synthesize glucan. These observations support the existence of catalytic subdomains containing glutamate and tryptophan (EAW) or aspartate and histidine (HDS) residues, each of which have been suggested to be involved with the catalytic activity of GTF. Furthermore, the epitopes defined in these sequences have significant immunogenicity and can induce immune responses which interfere with GTF-mediated glucan synthesis.

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^* Corresponding author. Mailing address: Department of Immunology, Forsyth Dental Center, 140 The Fenway, Boston, MA 02115. Phone: (617) 262-5200, ext. 309. Fax: (617) 262-4021. E-mail: dsmith{at}forsyth.org.

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Infection and Immunity, May 1999, p. 2638-2642, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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