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Infection and Immunity, May 1999, p. 2671-2676, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Cellular Form of Human Fibronectin as an Adhesion Target for the S Fimbriae of Meningitis-Associated Escherichia coli

Anne Sarén,1 Ritva Virkola,1,* Jörg Hacker,2 and Timo K. Korhonen1

Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland,1 and Institut für Molekulare Infektionsbiologie, Universität Würzburg, D-97070 Würzburg, Germany2

Received 5 October 1998/Returned for modification 25 November 1998/Accepted 10 February 1999

The adhesion of the S fimbriae of meningitis-associated Escherichia coli O18ac:K1:H7 to the cellular and the plasma forms of human fibronectin was studied. E. coli HB101(pAZZ50) expressing the complete S-fimbria II gene cluster of E. coli O18 adhered to cellular fibronectin (cFn) on glass but not to plasma fibronectin (pFn). Adhesion to cFn was specifically inhibited by neuraminidase treatment of cFn as well as by incubation of the bacteria with sialyl-alpha 2-3-lactose, a receptor analog of the S fimbriae. No significant adhesion to cFn or pFn was detected with E. coli HB101(pAZZ50-67) expressing S fimbriae lacking the SfaS lectin subunit. Strain HB101(pAZZ50) also adhered to a human fibroblast cell culture known to be rich in cFn, and the adhesion was specifically inhibited in the presence of polyclonal antibodies to cFn. The results show that the SfaS lectin of the S fimbriae mediates the adherence of meningitis-associated E. coli to sialyl oligosaccharide chains of cFn.

* Corresponding author. Mailing address: Division of General Microbiology, Department of Biosciences, P.O. Box 56, Viikinkaari 9, FIN-00014 University of Helsinki, Finland. Phone: 358-9-70859235. Fax: 358-9-70859262. E-mail: ritva.virkola{at}helsinki.fi.

Infection and Immunity, May 1999, p. 2671-2676, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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