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Infection and Immunity, May 1999, p. 2677-2681, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mechanisms of Internalization of Staphylococcus aureus by Cultured Human Osteoblasts

Marc Jevon,1 Chuanbin Guo,1,dagger Beechai Ma,1 Nicky Mordan,2 Sean P. Nair,1 Malcolm Harris,1 Brian Henderson,1 George Bentley,3 and Sajeda Meghji1,*

Maxillofacial Surgery Research Unit1 and Electron Microscopy Unit,2 Eastman Dental Institute, University College London, London, and Institute of Orthopaedics, Royal National Orthopaedic Hospital Trust, Stanmore, Middlesex,3 United Kingdom

Received 16 November 1998/Returned for modification 31 December 1998/Accepted 5 February 1999

Staphylococcus aureus is an important bone pathogen, and evidence shows that this organism is internalized by chick osteoblasts. Here we report that S. aureus is internalized by human osteoblasts. Internalization was inhibited by monodansylcadaverine and cytochalasin D and to a lesser extent by ouabain, monensin, colchicine, and nocodazole. We propose that internalization occurs via a receptor-mediated pathway, requiring the participation of cytoskeletal elements, principally actin.

* Corresponding author. Mailing address: Department of Oral and Maxillofacial Surgery, Eastman Dental Institute, 256 Gray's Inn Road, London, WC1X 8LD, United Kingdom. Phone and Fax: 0181 915 1271. E-mail: S.Meghji{at}eastman.ucl.ac.uk.

dagger Present address: Department of Oral and Maxillofacial Surgery, School of Stomatology, Beijing Medical University, Beijing 100081, Peoples Republic of China.

Infection and Immunity, May 1999, p. 2677-2681, Vol. 67, No. 5
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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