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Infection and Immunity, June 1999, p. 2763-2768, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Suppression of Platelet Aggregation by Bordetella pertussis Adenylate Cyclase Toxin

Masaaki Iwaki,1,* Kazunari Kamachi,1 Nikolaus Heveker,2,dagger and Toshifumi Konda1

Department of Bacterial and Blood Products, National Institute of Infectious Diseases, Musashimurayama-shi, Tokyo 208-0011, Japan,1 and Unité de Biochimie des Régulations Cellulaires, Institut Pasteur, 75724 Paris Cedex 15, France2

Received 21 December 1998/Returned for modification 5 February 1999/Accepted 16 March 1999

The effect of Bordetella pertussis adenylate cyclase toxin (ACT) on platelet aggregation was investigated. This cell-invasive adenylate cyclase completely suppressed ADP (10 µM)-induced aggregation of rabbit platelets at 3 µg/ml and strongly suppressed thrombin (0.2 U/ml)-induced aggregation at 10 µg/ml. The suppression was accompanied by marked increase in platelet intracellular cyclic AMP (cAMP) content and was diminished by the anti-ACT monoclonal antibody B7E11. A catalytically inactive point mutant of ACT did not show the suppressive effect. Since an increase of cAMP content is a known cause of platelet dysfunction, these results indicate that the observed platelet inactivation was due to the catalytic activity of ACT through increase of intracellular cAMP.

* Corresponding author. Mailing address: Department of Bacterial and Blood Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama-shi, Tokyo 208-0011, Japan. Phone: 81-42-561-0771, ext. 363. Fax: 81-42-561-7173. E-mail: miwaki{at}nih.go.jp.

dagger Present address: INSERM U 332, Institut Cochin de Génétique Moléculaire, 75014 Paris, France.

Infection and Immunity, June 1999, p. 2763-2768, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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