This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fan, X.
Right arrow Articles by Schütt, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fan, X.
Right arrow Articles by Schütt, C.

 Previous Article  |  Next Article 

Infection and Immunity, June 1999, p. 2964-2968, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Structures in Bacillus subtilis Are Recognized by CD14 in a Lipopolysaccharide Binding Protein-Dependent Reaction

Xiaolong Fan,1,dagger Felix Stelter,1,* Rene Menzel,1 Robert Jack,1 Ingo Spreitzer,2 Thomas Hartung,2 and Christine Schütt1

Institute of Immunology and Transfusion Medicine, D-17489 Greifswald,1 and Department of Biochemical Pharmacology, University of Konstanz, 78434 Konstanz,2 Germany

Received 15 October 1998/Returned for modification 23 November 1998/Accepted 24 March 1999

The CD14 molecule expressed on monocytes and macrophages is a high-affinity receptor for bacterial lipopolysaccharide (LPS) and hence an important component of the innate immune system. LPS binding protein (LBP) is required to facilitate the binding of LPS to CD14 in vitro and is necessary for the induction of an inflammatory response to LPS in vivo. Here we show that CD14 and LBP can also bind to lipoteichoic acid from the gram-positive bacterium Bacillus subtilis. Although CD14 does not interact with intact B. subtilis organisms, a brief exposure of the bacteria to serum converts them into a form which can bind to CD14 in an LBP-dependent reaction. When serum-pretreated B. subtilis organisms are incubated with the myelomonocytic cell line U937, which expresses CD14, the bacteria are rapidly phagocytosed. The phagocytosis is strictly dependent both on LBP and on CD14. These in vitro results suggest that LBP plays a role in the innate response not only to gram-negative but also to gram-positive infections.

* Corresponding author. Mailing address: Ernst-Moritz-Arndt-University Greifswald, Institute for Immunology and Transfusion Medicine, Klinikum, Sauerbruchstraße, D-17487 Greifswald, Germany. Phone: 49-3834-865455. Fax: 49-3834-865490. E-mail: stelter{at}uni-greifswald.de.

dagger Present address: Department of Molecular Medicine and Gene Therapy, Lund University, 223 62 Lund, Sweden.

Infection and Immunity, June 1999, p. 2964-2968, Vol. 67, No. 6
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Oliva, C., Turnbough, C. L. Jr., Kearney, J. F. (2009). CD14-Mac-1 interactions in Bacillus anthracis spore internalization by macrophages. Proc. Natl. Acad. Sci. USA 106: 13957-13962 [Abstract] [Full Text]  
  • Zeng, R., Bequette, B. J., Vinyard, B. T., Bannerman, D. D. (2009). Determination of milk and blood concentrations of lipopolysaccharide-binding protein in cows with naturally acquired subclinical and clinical mastitis. J DAIRY SCI 92: 980-989 [Abstract] [Full Text]  
  • Knapp, S., von Aulock, S., Leendertse, M., Haslinger, I., Draing, C., Golenbock, D. T., van der Poll, T. (2008). Lipoteichoic Acid-Induced Lung Inflammation Depends on TLR2 and the Concerted Action of TLR4 and the Platelet-Activating Factor Receptor. J. Immunol. 180: 3478-3484 [Abstract] [Full Text]  
  • Hermann, C. (2007). Review: Variability of host pathogen interaction. Innate Immunity 13: 199-218 [Abstract]  
  • Spiller, S., Dreher, S., Meng, G., Grabiec, A., Thomas, W., Hartung, T., Pfeffer, K., Hochrein, H., Brade, H., Bessler, W., Wagner, H., Kirschning, C. J. (2007). Cellular Recognition of Trimyristoylated Peptide or Enterobacterial Lipopolysaccharide via Both TLR2 and TLR4. J. Biol. Chem. 282: 13190-13198 [Abstract] [Full Text]  
  • Schroder, N. W.J., Schumann, R. R. (2005). Non-LPS targets and actions of LPS binding protein (LBP). Innate Immunity 11: 237-242 [Abstract]  
  • Branger, J., Florquin, S., Knapp, S., Leemans, J. C., Pater, J. M., Speelman, P., Golenbock, D. T., van der Poll, T. (2004). LPS-binding protein-deficient mice have an impaired defense against Gram-negative but not Gram-positive pneumonia. Int Immunol 16: 1605-1611 [Abstract] [Full Text]  
  • Neuhaus, F. C., Baddiley, J. (2003). A Continuum of Anionic Charge: Structures and Functions of D-Alanyl-Teichoic Acids in Gram-Positive Bacteria. Microbiol. Mol. Biol. Rev. 67: 686-723 [Abstract] [Full Text]  
  • Peters, K., Unger, R. E., Brunner, J., Kirkpatrick, C.J. (2003). Molecular basis of endothelial dysfunction in sepsis. Cardiovasc Res 60: 49-57 [Abstract] [Full Text]  
  • Muroi, M., Ohnishi, T., Azumi-Mayuzumi, S., Tanamoto, K.-i. (2003). Lipopolysaccharide-Mimetic Activities of a Toll-Like Receptor 2-Stimulatory Substance(s) in Enterobacterial Lipopolysaccharide Preparations. Infect. Immun. 71: 3221-3226 [Abstract] [Full Text]  
  • Schroder, N. W. J., Morath, S., Alexander, C., Hamann, L., Hartung, T., Zahringer, U., Gobel, U. B., Weber, J. R., Schumann, R. R. (2003). Lipoteichoic Acid (LTA) of Streptococcus pneumoniae and Staphylococcus aureus Activates Immune Cells via Toll-like Receptor (TLR)-2, Lipopolysaccharide-binding Protein (LBP), and CD14, whereas TLR-4 and MD-2 Are Not Involved. J. Biol. Chem. 278: 15587-15594 [Abstract] [Full Text]  
  • Vreugdenhil, A. C. E., Rousseau, C. H., Hartung, T., Greve, J. W. M., van 't Veer, C., Buurman, W. A. (2003). Lipopolysaccharide (LPS)-Binding Protein Mediates LPS Detoxification by Chylomicrons. J. Immunol. 170: 1399-1405 [Abstract] [Full Text]  
  • Morath, S., Geyer, A., Spreitzer, I., Hermann, C., Hartung, T. (2002). Structural Decomposition and Heterogeneity of Commercial Lipoteichoic Acid Preparations. Infect. Immun. 70: 938-944 [Abstract] [Full Text]  
  • Diterich, I., Harter, L., Hassler, D., Wendel, A., Hartung, T. (2001). Modulation of Cytokine Release in Ex Vivo-Stimulated Blood from Borreliosis Patients. Infect. Immun. 69: 687-694 [Abstract] [Full Text]  
  • Schroder, N. W. J., Opitz, B., Lamping, N., Michelsen, K. S., Zahringer, U., Gobel, U. B., Schumann, R. R. (2000). Involvement of Lipopolysaccharide Binding Protein, CD14, and Toll-Like Receptors in the Initiation of Innate Immune Responses by Treponema Glycolipids. J. Immunol. 165: 2683-2693 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»