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Infection and Immunity, July 1999, p. 3399-3402, Vol. 67, No. 7
Division of Microbiology, National Institute
of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan
Received 12 October 1998/Returned for modification 24 November
1998/Accepted 6 April 1999
Lipopolysaccharide (LPS) obtained from Porphyromonas
gingivalis was found to exhibit marked lethal toxicity in
galactosamine-sensitized C3H/HeJ mice. Although no lethality was
observed in mice intraperitoneally challenged with 1 mg of P. gingivalis LPS without galactosamine, when they were sensitized
with 30 mg of galactosamine, challenge with 1 and 10 µg of LPS
resulted in 67 and 100% lethality, respectively. The lethal dose of
LPS was almost the same in LPS-responsive C57BL/6 mice and
non-LPS-responsive C3H/HeJ mice. Furthermore, when 1 µg of P. gingivalis LPS was administered to each mouse 90 min before the
challenge with the same LPS with galactosamine, tolerance to the lethal
action of LPS was induced, and the mice were completely protected from
death, even at a dose 100-fold greater than the lethal dose of LPS.
Neither a lethal effect nor induction of tolerance to the lethality of
P. gingivalis LPS was exhibited by Salmonella LPS in galactosamine-sensitized C3H/HeJ mice. A protein-LPS complex derived from Pseudomonas aeruginosa, which exhibited strong
lethality and induced tolerance to a subsequent challenge with a lethal dose of LPS in galactosamine-sensitized LPS-responsive mice, did not
exhibit lethal toxicity in galactosamine-sensitized C3H/HeJ mice and
failed to induce tolerance in these mice to the lethality of P. gingivalis LPS. These results indicate that P. gingivalis LPS plays the central role in the activation of
non-LPS-responsive C3H/HeJ mice.
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Induction of Lethal Shock and Tolerance by Porphyromonas
gingivalis Lipopolysaccharide in
D-Galactosamine-Sensitized C3H/HeJ Mice
*
Mailing address: Division of Microbiology, National
Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo
158-8501, Japan. Phone: 81-3-3700-1141, ext. 272. Fax: 81-3-3707-6950. E-mail: tanamoto{at}nihs.go.jp.
Infection and Immunity, July 1999, p. 3399-3402, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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