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Infection and Immunity, July 1999, p. 3645-3648, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Antibody-Based Protection of Gnotobiotic Piglets Infected with Escherichia coli O157:H7 against Systemic Complications Associated with Shiga Toxin 2

Art Donohue-Rolfe, Ivanela Kondova, Jean Mukherjee, Kerry Chios, David Hutto, and Saul Tzipori*

Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536

Received 3 March 1999/Returned for modification 5 April 1999/Accepted 13 April 1999

Hemolytic-uremic syndrome (HUS) is a serious disease in children, attributable in the majority of cases to infection with Shiga toxin (Stx)-producing Escherichia coli. Using gnotobiotic piglets orally infected with E. coli O157:H7, which develop Stx-related cerebellar lesions and fatal neurological symptoms, we show that administration of Stx2-specific antiserum well after challenge protected, in a dose-response fashion, against these symptoms for at least 24 h after bacterial challenge. Twenty-six of 30 piglets given Stx2 antiserum survived the challenge, compared to only 4 of 16 animals given control serum or saline. Given our observations in piglets, Stx antibody of human origin may likewise prevent HUS in children.

* Corresponding author. Mailing address: Division of Infectious Diseases, Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536. Phone: (508) 839-7955. Fax: (508) 839-7977. E-mail: Stzipori{at}infonet.tufts.edu.

Infection and Immunity, July 1999, p. 3645-3648, Vol. 67, No. 7
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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