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Infection and Immunity, August 1999, p. 3733-3739, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Vibrio cholerae Intestinal Population Dynamics in the Suckling Mouse Model of Infection

Michael J. Angelichio,1 Jonathon Spector,2 Matthew K. Waldor,2,* and Andrew Camilli1,*

Department of Molecular Biology and Microbiology, Tufts University School of Medicine,1 and Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center,2 Boston, Massachusetts 02111

Received 19 January 1999/Returned for modification 29 March 1999/Accepted 27 April 1999

The suckling mouse has been used as a model to identify Vibrio cholerae intestinal colonization factors for over two decades, yet little is known about the location of recoverable organisms along the gastrointestinal (GI) tract following intragastric inoculation. In the present study, we determined the population dynamics of wild-type and avirulent mutant derivatives of both classical and El Tor biotype strains throughout the entire suckling mouse GI tract at various times after intragastric inoculation. Wild-type strains preferentially colonized the middle small bowel with a sharp demarcation between more proximal segments which had manyfold-fewer recoverable cells. Surprisingly, large and stable populations of viable cells were also recovered from the cecum and large bowel. Strains lacking toxin-coregulated pili (TCP-) were cleared from the small bowel; however, an El Tor TCP- strain colonized the cecum and large bowel almost as well as the wild-type strain. Strains lacking lipopolysaccharide O antigen (OA-) were efficiently cleared from the small bowel at early times but then showed net growth for the remainder of the infections. Moreover, large populations of the OA- strains were maintained in the large bowel. These results show that for the El Tor biotype neither TCP nor OA is required for colonization of the suckling mouse large bowel. Finally, similar percent recoveries of wild-type, TCP-, and OA- strains from the small bowel at an early time after infection suggest that TCP and OA are not required for strains of either biotype to resist bactericidal mechanisms in the suckling mouse GI tract.

* Corresponding author. Mailing address for Matthew K. Waldor: Division of Geographic Medicine and Infectious Diseases, Tufts-New England Medical Center, 750 Washington St., Boston, MA 02111. Phone: (617) 636-7618. Fax: (617) 636-5292. E-mail: matthew.waldor{at}es.nemc.org. Mailing address for Andrew Camilli: Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-6653. Fax: (617) 636-0337. E-mail: acamilli{at}opal.tufts.edu.

Infection and Immunity, August 1999, p. 3733-3739, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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