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Infection and Immunity, August 1999, p. 3847-3854, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Adhesion of Tritrichomonas foetus to Bovine Vaginal Epithelial Cells

B. N. Singh,1,* J. J. Lucas,1 D. H. Beach,2 S. T. Shin,3,dagger and R. O. Gilbert3

Department of Biochemistry and Molecular Biology1 and Department of Microbiology and Immunology,2 SUNY Health Science Center, Syracuse, New York 13210, and Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York 148533

Received 17 December 1998/Returned for modification 18 February 1999/Accepted 12 May 1999

An in vitro culture system of bovine vaginal epithelial cells (BVECs) was developed to study the cytopathogenic effects of Tritrichomonas foetus and the role of lipophosphoglycan (LPG)-like cell surface glycoconjugates in adhesion of parasites to host cells. Exposure of BVEC monolayers to T. foetus resulted in extensive damage of monolayers. Host cell disruption was measured quantitatively by a trypan blue exclusion assay and by release of 3H from [3H]thymidine-labeled host cells. Results indicated contact-dependent cytotoxicity of host cells by T. foetus. The cytopathogenic effect was a function of T. foetus density. Metronidazole- or periodate-treated T. foetus showed no damage to BVEC monolayers. A related human trichomonad, Trichomonas vaginalis, showed no cytotoxic effects, indicating species-specific host-parasite interactions. A direct binding assay was developed and used to investigate the role of a major cell surface LPG-like molecule in host-parasite adhesion. The results of competition experiments showed that the binding to BVECs was displaceable, was saturable, and yielded a typical binding curve, suggesting that specific receptor-ligand interactions mediate the attachment of T. foetus to BVECs. Progesterone-treated BVECs showed enhanced parasite binding. T. foetus LPG inhibited the binding of T. foetus to BVECs; the LPG from T. vaginalis and a variety of other glycoconjugates did not. These data imply specificity of LPG on host-parasite adhesion. Periodate-treated parasites showed no adherence to host cells, indicating the involvement of carbohydrate containing molecules in the adhesion process.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, SUNY Health Science Center, Syracuse, NY 13210. Phone: (315) 464-5398. Fax: (315) 464-8750. E-mail: Singhb{at}vax.cs.hscsyr.edu.

dagger Present address: Department of Veterinary Medicine, Chungnam National University, Taejon 305-764, Korea.

Infection and Immunity, August 1999, p. 3847-3854, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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