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Infection and Immunity, August 1999, p. 4014-4018, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

New Exfoliative Toxin Produced by a Plasmid-Carrying Strain of Staphylococcus hyicus

Hisaaki Sato,1,* Takao Watanabe,1 Yasuko Murata,1 Ayumi Ohtake,1 Mayumi Nakamura,1 Chikara Aizawa,2 Hiroshi Saito,2 and Nobutoshi Maehara1

Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034,1 and Department of Virology, The Kitasato Institute, Minato-ku, Tokyo 108,2 Japan

Received 14 October 1998/Returned for modification 9 December 1998/Accepted 9 April 1999

A new serotype of Staphylococcus hyicus exfoliative toxin (SHET), serotype B, was isolated from the culture filtrate of a plasmid-carrying strain of S. hyicus. The new SHET was purified by precipitation with 70% saturated ammonium sulfate, gel filtration on a Sephadex G-75 column, column chromatography on DEAE-Cellulofine A-500, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The new SHET caused exfoliation of the epidermis as determined by the so-called Nikolsky sign when inoculated into 1-day-old chickens. The new SHET was serologically different from Staphylococcus aureus exfoliative toxins (ETs) (ETA, ETB, and ETC) and from the SHET from the plasmidless strain but showed the same molecular weight as the other serotypes of toxins on SDS-PAGE. It was thermolabile and lost its toxicity after being heated at 60°C for 30 min. We propose that the new SHET be designated SHETB and that the SHET produced by the plasmidless strain be designated SHETA.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034, Japan. Phone: 0176-23-4371. Fax: 0176-23-8703. E-mail: satoh{at}vmas.kitasato-u.ac.jp.

Infection and Immunity, August 1999, p. 4014-4018, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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