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Infection and Immunity, August 1999, p. 4027-4032, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

A Murine Model for Infection with Burkholderia cepacia with Sustained Persistence in the Spleen

David P. Speert,1,2,3,* Barbara Steen,2 Keith Halsey,1 and Eddie Kwan1

Division of Infectious and Immunological Diseases, Department of Pediatrics,1 Department of Microbiology and Immunology,2 and the Centre for Microbial Diseases and Host Defence Research,3 University of British Columbia, Vancouver, British Columbia, Canada V5Z 4H4

Received 28 January 1999/Returned for modification 15 April 1999/Accepted 10 May 1999

Burkholderia cepacia is an opportunistic pathogen that causes severe systemic infections in patients with chronic granulomatous disease (CGD) or with cystic fibrosis (CF), but its mechanisms of virulence are poorly understood. We developed a murine model of systemic infection in wild-type (WT) and gamma interferon knockout (GKO) BALB/c mice to facilitate dissection of components of pathogenicity and host defense. Both WT and GKO mice were susceptible to chronic splenic infection with B. cepacia, but not with Pseudomonas aeruginosa. B. cepacia strains from patients with CGD persisted longer than those from CF patients. C57BL/6 mice were the most susceptible murine strain; bacteria persisted in the spleen for 2 months. DBA/2, BALB/c, and A/J strains of mice were relatively resistant to infection. Certain strains of B. cepacia complex can persist in the murine spleen after systemic infection; this may provide clues to its virulence in compromised hosts, such as those with CGD and CF.

* Corresponding author. Mailing address: BC Research Institute for Children's & Women's Health, Room 377, 950 West 28th Ave., Vancouver, B.C., Canada V5Z 4H4. Phone: (604) 875-2438. Fax: (604) 875-2226. E-mail: speert{at}unixg.ubc.ca.

Infection and Immunity, August 1999, p. 4027-4032, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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