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Infection and Immunity, August 1999, p. 4216-4222, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Heat-Shocked Monocytes Are Resistant to Staphylococcus aureus-Induced Apoptotic DNA Fragmentation due to Expression of HSP72

Krzysztof Guzik,1 Malgorzata Bzowska,1 Jerzy Dobrucki,2 and Juliusz Pryjma1,*

Department of Microbiology and Immunology1 and Department of Biophysics,2 Institute of Molecular Biology, Jagiellonian University, Cracow, Poland

Received 18 February 1999/Returned for modification 17 March 1999/Accepted 17 May 1999

Human peripheral blood monocytes became apoptotic following phagocytosis of Staphylococcus aureus. The consequences of heat stress for monocytes were studied with regard to the effect on S. aureus-induced apoptosis. Exposure of monocytes to 41.5°C for 1 h resulted in HSP72 expression and had no influence on phagocytosis of bacteria; moreover, phagocytosis of S. aureus immediately or shortly after heat shock had no effect on the S. aureus-induced monocyte apoptosis, as evidenced by DNA fragmentation assay. In contrast, cells which recovered from heat shock for 18 to 24 h, although active as phagocytes, were resistant to the S. aureus-induced apoptosis. The observed protective effect was related to the induction of HSP72, since blocking of HSP72 synthesis by an antisense oligomer abolished the protective effect of heat shock on bacterium-induced monocyte apoptosis.

* Corresponding author. Mailing address. Jagiellonian University, Institute of Molecular Biology, Al. Mickiewicza 3, 31-120 Cracow, Poland. Phone: (48-12) 6341305, ext. 258. Fax: (48-12) 6336907. E-mail: PRYJMA{at}mol.uj.edu.pl.

Infection and Immunity, August 1999, p. 4216-4222, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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