This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoyer, L. L.
Right arrow Articles by Poulet, F. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoyer, L. L.
Right arrow Articles by Poulet, F. M.

 Previous Article  |  Next Article 

Infection and Immunity, August 1999, p. 4251-4255, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Als Proteins on the Cell Wall of Candida albicans in Murine Tissues

L. L. Hoyer,* J. Clevenger, J. E. Hecht, E. J. Ehrhart, and F. M. Pouletdagger

Department of Veterinary Pathobiology, University of Illinois, Urbana, Illinois

Received 23 February 1999/Returned for modification 19 March 1999/Accepted 11 May 1999

A murine model of disseminated candidiasis was utilized to determine whether Candida albicans Als proteins are produced in vivo. The kidneys, spleen, heart, liver, and lungs were collected from mice inoculated with one of three C. albicans strains (SC5314, B311, or WO-1). Immunohistochemical analysis of murine tissues by using a rabbit polyclonal anti-Als serum indicated that Als proteins were produced by each C. albicans cell in the tissues examined. Patterns of staining with the anti-Als serum were similar among the C. albicans strains tested. These data indicated that Als protein production was widespread in disseminated candidiasis and that, despite strain differences in ALS gene expression previously noted in vitro, Als protein production in vivo was similar among C. albicans strains. The extensive production of Als proteins in vivo and their presence on the C. albicans cell wall position these proteins well for a role in host-pathogen interaction.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign, 2522 VMBSB, 2001 S. Lincoln Ave., Urbana, IL 61802. Phone: (217) 333-5043. Fax: (217) 244-7421. E-mail: lhoyer{at}uiuc.edu.

dagger Present address: Schering-Plough Research Institute, Lafayette, NJ 07848.

Infection and Immunity, August 1999, p. 4251-4255, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Fradin, C., Slomianny, M. C., Mille, C., Masset, A., Robert, R., Sendid, B., Ernst, J. F., Michalski, J. C., Poulain, D. (2008). {beta}-1,2 Oligomannose Adhesin Epitopes Are Widely Distributed over the Different Families of Candida albicans Cell Wall Mannoproteins and Are Associated through both N- and O-Glycosylation Processes. Infect. Immun. 76: 4509-4517 [Abstract] [Full Text]  
  • Green, C. B., Zhao, X., Hoyer, L. L. (2005). Use of Green Fluorescent Protein and Reverse Transcription-PCR To Monitor Candida albicans Agglutinin-Like Sequence Gene Expression in a Murine Model of Disseminated Candidiasis. Infect. Immun. 73: 1852-1855 [Abstract] [Full Text]  
  • Weig, M., Jansch, L., Gross, U., De Koster, C. G., Klis, F. M., De Groot, P. W. J. (2004). Systematic identification in silico of covalently bound cell wall proteins and analysis of protein-polysaccharide linkages of the human pathogen Candida glabrata. Microbiology 150: 3129-3144 [Abstract] [Full Text]  
  • Sheppard, D. C., Yeaman, M. R., Welch, W. H., Phan, Q. T., Fu, Y., Ibrahim, A. S., Filler, S. G., Zhang, M., Waring, A. J., Edwards, J. E. Jr. (2004). Functional and Structural Diversity in the Als Protein Family of Candida albicans. J. Biol. Chem. 279: 30480-30489 [Abstract] [Full Text]  
  • Bernard, M., Mouyna, I., Dubreucq, G., Debeaupuis, J.-P., Fontaine, T., Vorgias, C., Fuglsang, C., Latge, J.-P. (2002). Characterization of a cell-wall acid phosphatase (PhoAp) in Aspergillus fumigatus. Microbiology 148: 2819-2829 [Abstract] [Full Text]  
  • Glee, P. M., Cutler, J. E., Benson, E. E., Bargatze, R. F., Hazen, K. C. (2001). Inhibition of Hydrophobic Protein-Mediated Candida albicans Attachment to Endothelial Cells during Physiologic Shear Flow. Infect. Immun. 69: 2815-2820 [Abstract] [Full Text]  
  • Timpel, C., Zink, S., Strahl-Bolsinger, S., Schröppel, K., Ernst, J. (2000). Morphogenesis, Adhesive Properties, and Antifungal Resistance Depend on the Pmt6 Protein Mannosyltransferase in the Fungal Pathogen Candida albicans. J. Bacteriol. 182: 3063-3071 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»