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Infection and Immunity, August 1999, p. 4256-4259, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Differences in the Concentrations of Small, Anionic, Antimicrobial Peptides in Bronchoalveolar Lavage Fluid and in Respiratory Epithelia of Patients with and without Cystic Fibrosis

Kim A. Brogden,1,* Mark R. Ackermann,2 Paul B. McCray Jr.,3 and Kenneth M. Huttner4

Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 500101; Department of Veterinary Pathology, Iowa State University, Ames, Iowa 500112; Department of Pediatrics, Allergy/Pulmonary Division, The University of Iowa Hospitals and Clinics, Iowa City, Iowa 52242-10833; and Joint Program in Neonatology, Department of Medicine, Boston Children's Hospital, Boston, Massachusetts 021154

Received 18 November 1998/Returned for modification 5 January 1999/Accepted 17 May 1999

Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2) antibodies to synthetic H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant, were prepared and used to assess the concentrations of AP-like molecules in human respiratory tract samples. In bronchoalveolar lavage fluids, concentrations of AP-like molecules measured by enzyme-linked immunosorbent assay were significantly lower in 13 patients with cystic fibrosis (CF) (mean ± standard deviation [SD], 0.78 ± 0.46 mM) than in 34 patients without CF (1.30 ± 0.66 mM) (P = 0.01). In pulmonary tissues of three patients without CF, very little antigen was stained in the apical cytoplasm of the bronchial and bronchiolar epithelium yet robust staining was seen in the alveolar epithelium. In pulmonary tissues of three patients with CF, robust staining of antigen was seen in the apical cytoplasm of the bronchial and bronchiolar epithelium yet no staining was seen in the alveolar epithelium. These results show that AP-like molecules are present in healthy human respiratory tract samples and differ in concentration and location of expression in patients with and without CF.

* Corresponding author. Mailing address: Respiratory and Neurologic Disease Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 70, 2300 Dayton Rd., Ames, IA 50010. Phone: (515) 239-8593. Fax: (515) 239-8458. E-mail: kbrogden{at}nadc.ars.usda.gov.

Infection and Immunity, August 1999, p. 4256-4259, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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