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Infection and Immunity, August 1999, p. 4280-4284, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Avirulence of Candida albicans CaHK1 Mutants in a Murine Model of Hematogenously Disseminated Candidiasis

José Antonio Calera,1,* Xiao-Jiong Zhao,1 Flavia De Bernardis,2 Michael Sheridan,3 and Richard Calderone1

Department of Microbiology and Immunology, Georgetown University Medical Center, Washington, D.C. 20007-21971; Laboratory of Bacteriology and Medical Mycology, Istituto Superiore di Sanita, 00161 Rome, Italy2; and Division of Epidemiology and Statistics, Institute of Research and Education, INOVA Health System, Falls Church, Virginia 220463

Received 10 February 1999/Returned for modification 22 April 1999/Accepted 28 May 1999

Deletion of both alleles of the Candida albicans CaHK1 gene, which causes cells to flocculate when grown at pH 7.5, a pH comparable to that of mammalian blood, abolishes the ability of the yeast to establish a successful infection in a murine model of hematogenously disseminated candidiasis. Within 72 h all mice inoculated with the parental C. albicans strain had died. The mice infected with either the heterozygote or revertant strain, either of which harbors only one functional CaHK1 allele, also succumbed to the infection, although survivors were observed for up to 16 days postinfection. However, mice inoculated with the Delta cahk1 null strain survived for the course of the infection. These results indicate that CaHK1 is required for the virulence of C. albicans in a murine model of hematogenously disseminated candidiasis. In contrast, CaHK1 is not required for the virulence of C. albicans in a rat model of vaginal candidiasis.

* Corresponding author. Mailing address: Department of Microbiology, SE303 Med-Dent Bldg., Georgetown University Medical Center, 3900 Reservoir Rd., N.W., Washington, DC 20007-2197. Phone: (202) 687-1796. Fax: (202) 687-1800. E-mail: abadj{at}gusun.georgetown.edu.

Infection and Immunity, August 1999, p. 4280-4284, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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