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Infection and Immunity, August 1999, p. 4290-4294, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Expression and Immunogenicity of a Mutant Diphtheria Toxin Molecule, CRM197, and Its Fragments in Salmonella typhi Vaccine Strain CVD 908-htrA

Nadav Orr,dagger James E. Galen, and Myron M. Levine*

Department of Pediatrics, Division of Infectious Diseases and Tropical Pediatrics, Center for Vaccine Development, and Department of Medicine, Division of Geographic Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201

Received 1 February 1999/Returned for modification 24 March 1999/Accepted 30 April 1999

Mutant diphtheria toxin molecule CRM197 and fragments thereof were expressed in attenuated Salmonella typhi CVD 908-htrA, and the constructs were tested for their ability to induce serum antitoxin. Initially, expressed proteins were insoluble, and the constructs failed to induce neutralizing antitoxin. Soluble CRM197 was expressed at low levels by utilizing the hemolysin A secretion system from Escherichia coli.

* Corresponding author. Mailing address: Center for Vaccine Development, University of Maryland School of Medicine, 685 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706-7588. Fax: (410) 706-6205. E-mail: MLEVINE{at}UMPPA1.AB.UMD.EDU.

dagger Present address: Army Health Branch Research Unit, Israel Defense Force, Israel.

Infection and Immunity, August 1999, p. 4290-4294, Vol. 67, No. 8
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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