This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murphy, T. F.
Right arrow Articles by Sethi, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murphy, T. F.
Right arrow Articles by Sethi, S.

 Previous Article  |  Next Article 

Infection and Immunity, September 1999, p. 4578-4585, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Analysis of Antigenic Structure and Human Immune Response to Outer Membrane Protein CD of Moraxella catarrhalis

Timothy F. Murphy,1,2,3,* Charmaine Kirkham,1 Ernesto DeNardin,4 and Sanjay Sethi3,5

Divisions of Infectious Diseases1 and Pulmonary and Critical Care Medicine,5 Department of Medicine, and Department of Microbiology,2 School of Medicine and Biomedical Sciences, and Department of Oral Biology, School of Dental Medicine,4 State University of New York at Buffalo, and the Veterans Affairs Western New York Healthcare System,3 Buffalo, New York 14215

Received 16 April 1999/Returned for modification 21 May 1999/Accepted 21 June 1999

Moraxella catarrhalis is an important cause of otitis media in children and lower respiratory tract infections in adults with chronic obstructive pulmonary disease (COPD). Outer membrane protein CD (OMP CD) is a 45-kDa protein which is a potential vaccine antigen to prevent infections caused by M. catarrhalis. Eight monoclonal antibodies were used to study the antigenic structure of the OMP CD molecule by assaying recombinant peptides corresponding to the sequence of the protein. This approach identified two surface-exposed epitopes, including one near the amino terminus (amino acids 25 to 44) and one in the central region of the molecule (amino acids 261 to 331). Assays with serum and sputum supernatants of adults with COPD revealed variable levels of antibodies to OMP CD among individuals. To determine which portions of the OMP CD molecule were recognized by human antibodies, three human serum samples were studied with six recombinant peptides which span the sequence of OMP CD. All three sera contained immunoglobulin G antibodies which recognized exclusively the peptide corresponding to amino acids 203 to 260 by immunoblot assay. Adsorption experiments with whole bacteria established that some of the human antibodies are directed at surface-exposed epitopes on OMP CD. We conclude that OMP CD is a highly conserved molecule which contains at least two separate epitopes which are exposed on the bacterial surface. While individual adults with COPD show variability in the immune response to OMP CD, a specific region of the OMP CD molecule (amino acids 203 to 260) is important as a target of the human immune response.

* Corresponding author. Mailing address: Veterans Affairs Western New York Healthcare System, Medical Research 151, 3495 Bailey Ave., Buffalo, NY 14215. Phone: (716) 829-2173. Fax: (716) 862-6526. E-mail: murphyt{at}acsu.buffalo.edu.

Infection and Immunity, September 1999, p. 4578-4585, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Balder, R., Hassel, J., Lipski, S., Lafontaine, E. R. (2007). Moraxella catarrhalis Strain O35E Expresses Two Filamentous Hemagglutinin-Like Proteins That Mediate Adherence to Human Epithelial Cells. Infect. Immun. 75: 2765-2775 [Abstract] [Full Text]  
  • Liu, D.-F., McMichael, J. C., Baker, S. M. (2007). Moraxella catarrhalis Outer Membrane Protein CD Elicits Antibodies That Inhibit CD Binding to Human Mucin and Enhance Pulmonary Clearance of M. catarrhalis in a Mouse Model. Infect. Immun. 75: 2818-2825 [Abstract] [Full Text]  
  • Becker, P. D., Bertot, G. M., Souss, D., Ebensen, T., Guzman, C. A., Grinstein, S. (2007). Intranasal Vaccination with Recombinant Outer Membrane Protein CD and Adamantylamide Dipeptide as the Mucosal Adjuvant Enhances Pulmonary Clearance of Moraxella catarrhalis in an Experimental Murine Model. Infect. Immun. 75: 1778-1784 [Abstract] [Full Text]  
  • Lipski, S. L., Akimana, C., Timpe, J. M., Wooten, R. M., Lafontaine, E. R. (2007). The Moraxella catarrhalis Autotransporter McaP Is a Conserved Surface Protein That Mediates Adherence to Human Epithelial Cells through Its N-Terminal Passenger Domain. Infect. Immun. 75: 314-324 [Abstract] [Full Text]  
  • Edwards, K. J., Schwingel, J. M., Datta, A. K., Campagnari, A. A. (2005). Multiplex PCR Assay That Identifies the Major Lipooligosaccharide Serotype Expressed by Moraxella catarrhalis Clinical Isolates. J. Clin. Microbiol. 43: 6139-6143 [Abstract] [Full Text]  
  • Bullard, B., Lipski, S. L., Lafontaine, E. R. (2005). Hag Directly Mediates the Adherence of Moraxella catarrhalis to Human Middle Ear Cells. Infect. Immun. 73: 5127-5136 [Abstract] [Full Text]  
  • Holm, M. M., Vanlerberg, S. L., Foley, I. M., Sledjeski, D. D., Lafontaine, E. R. (2004). The Moraxella catarrhalis Porin-Like Outer Membrane Protein CD Is an Adhesin for Human Lung Cells. Infect. Immun. 72: 1906-1913 [Abstract] [Full Text]  
  • Murphy, T. F., Kirkham, C., Liu, D.-F., Sethi, S. (2003). Human Immune Response to Outer Membrane Protein CD of Moraxella catarrhalis in Adults with Chronic Obstructive Pulmonary Disease. Infect. Immun. 71: 1288-1294 [Abstract] [Full Text]  
  • Lieberman, D., Lieberman, D., Gelfer, Y., Varshavsky, R., Dvoskin, B., Leinonen, M., Friedman, M. G. (2002). Pneumonic vs Nonpneumonic Acute Exacerbations of COPD. Chest 122: 1264-1270 [Abstract] [Full Text]  
  • Verduin, C. M., Hol, C., Fleer, A., van Dijk, H., van Belkum, A. (2002). Moraxella catarrhalis: from Emerging to Established Pathogen. Clin. Microbiol. Rev. 15: 125-144 [Abstract] [Full Text]  
  • Sethi, S., Murphy, T. F. (2001). Bacterial Infection in Chronic Obstructive Pulmonary Disease in 2000: a State-of-the-Art Review. Clin. Microbiol. Rev. 14: 336-363 [Abstract] [Full Text]  
  • Lafontaine, E. R., Wagner, N. J., Hansen, E. J. (2001). Expression of the Moraxella catarrhalis UspA1 Protein Undergoes Phase Variation and Is Regulated at the Transcriptional Level. J. Bacteriol. 183: 1540-1551 [Abstract] [Full Text]  
  • Murphy, T. F., Brauer, A. L., Yuskiw, N., Hiltke, T. J. (2000). Antigenic Structure of Outer Membrane Protein E of Moraxella catarrhalis and Construction and Characterization of Mutants. Infect. Immun. 68: 6250-6256 [Abstract] [Full Text]  
  • Ofori-Darko, E., Zavros, Y., Rieder, G., Tarle, S. A., Van Antwerp, M., Merchant, J. L. (2000). An OmpA-Like Protein from Acinetobacter spp. Stimulates Gastrin and Interleukin-8 Promoters. Infect. Immun. 68: 3657-3666 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»