This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Snewin, V. A.
Right arrow Articles by Young, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Snewin, V. A.
Right arrow Articles by Young, D. B.

 Previous Article  |  Next Article 

Infection and Immunity, September 1999, p. 4586-4593, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Assessment of Immunity to Mycobacterial Infection with Luciferase Reporter Constructs

Valerie A. Snewin,* Marie-Pierre Gares, Peadar Ó Gaora, Zahra Hasan,dagger Ivor N. Brown, and Douglas B. Young

Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St. Mary's Campus, London W2 1PG, United Kingdom

Received 17 September 1998/Returned for modification 30 October 1998/Accepted 14 June 1999

Protective immunity to mycobacterial infection is incompletely understood but probably involves the coordinated interaction of multiple cell types and cytokines. With the aim of developing assays that might provide a surrogate measure of protective immunity, we have investigated the use of recombinant mycobacteria carrying luciferase reporter enzymes to assess the effectiveness of antimycobacterial immunity in model systems. Measurement of luminescence was shown to provide a rapid and simple alternative to the counting of CFU as a means of monitoring mycobacterial viability. We describe optimization of a luciferase reporter strain of Mycobacterium tuberculosis and demonstrate its application for the study of mycobacterial interactions with host cells in tissue culture and the rapid assessment of vaccine efficacy in a murine model.

* Corresponding author. Mailing address: Department of Infectious Diseases and Microbiology, Imperial College School of Medicine, St. Mary's Campus, Norfolk Place, London W2 1PG, United Kingdom. Phone: 44-171-594 3956. Fax: 44-171 262 6299. E-mail: v.snewin{at}ic.ac.uk.

dagger Present address: Department of Microbiology, The Aga Khan University, Karachi 74800, Pakistan.

Infection and Immunity, September 1999, p. 4586-4593, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Veyrier, F., Said-Salim, B., Behr, M. A. (2008). Evolution of the Mycobacterial SigK Regulon. J. Bacteriol. 190: 1891-1899 [Abstract] [Full Text]  
  • Newton, S. M., Mackie, S. L., Martineau, A. R., Wilkinson, K. A., Kampmann, B., Fisher, C., Dutta, S., Levin, M., Wilkinson, R. J., Pasvol, G. (2008). Reduction of Chemokine Secretion in Response to Mycobacteria in Infliximab-Treated Patients. CVI 15: 506-512 [Abstract] [Full Text]  
  • Reddy, V. M., Einck, L., Nacy, C. A. (2008). In Vitro Antimycobacterial Activities of Capuramycin Analogues. Antimicrob. Agents Chemother. 52: 719-721 [Abstract] [Full Text]  
  • Martineau, A. R., Wilkinson, R. J., Wilkinson, K. A., Newton, S. M., Kampmann, B., Hall, B. M., Packe, G. E., Davidson, R. N., Eldridge, S. M., Maunsell, Z. J., Rainbow, S. J., Berry, J. L., Griffiths, C. J. (2007). A Single Dose of Vitamin D Enhances Immunity to Mycobacteria. Am. J. Respir. Crit. Care Med. 176: 208-213 [Abstract] [Full Text]  
  • Martineau, A. R., Wilkinson, K. A., Newton, S. M., Floto, R. A., Norman, A. W., Skolimowska, K., Davidson, R. N., Sorensen, O. E., Kampmann, B., Griffiths, C. J., Wilkinson, R. J. (2007). IFN-{gamma}- and TNF-Independent Vitamin D-Inducible Human Suppression of Mycobacteria: The Role of Cathelicidin LL-37. J. Immunol. 178: 7190-7198 [Abstract] [Full Text]  
  • Cho, S. H., Warit, S., Wan, B., Hwang, C. H., Pauli, G. F., Franzblau, S. G. (2007). Low-Oxygen-Recovery Assay for High-Throughput Screening of Compounds against Nonreplicating Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 51: 1380-1385 [Abstract] [Full Text]  
  • Rosseels, V., Roupie, V., Zinniel, D., Barletta, R. G., Huygen, K. (2006). Development of Luminescent Mycobacterium avium subsp. paratuberculosis for Rapid Screening of Vaccine Candidates in Mice.. Infect. Immun. 74: 3684-3686 [Abstract] [Full Text]  
  • D'Souza, S., Romano, M., Korf, J., Wang, X.-M., Adnet, P.-Y., Huygen, K. (2006). Partial Reconstitution of the CD4+-T-Cell Compartment in CD4 Gene Knockout Mice Restores Responses to Tuberculosis DNA Vaccines.. Infect. Immun. 74: 2751-2759 [Abstract] [Full Text]  
  • Protopopova, M., Hanrahan, C., Nikonenko, B., Samala, R., Chen, P., Gearhart, J., Einck, L., Nacy, C. A. (2005). Identification of a new antitubercular drug candidate, SQ109, from a combinatorial library of 1,2-ethylenediamines. J Antimicrob Chemother 56: 968-974 [Abstract] [Full Text]  
  • Wiles, S., Ferguson, K., Stefanidou, M., Young, D. B., Robertson, B. D. (2005). Alternative Luciferase for Monitoring Bacterial Cells under Adverse Conditions. Appl. Environ. Microbiol. 71: 3427-3432 [Abstract] [Full Text]  
  • Blokpoel, M. C. J., Murphy, H. N., O'Toole, R., Wiles, S., Runn, E. S. C., Stewart, G. R., Young, D. B., Robertson, B. D. (2005). Tetracycline-inducible gene regulation in mycobacteria. Nucleic Acids Res 33: e22-e22 [Abstract] [Full Text]  
  • Kampmann, B., Tena, G. N., Mzazi, S., Eley, B., Young, D. B., Levin, M. (2004). Novel Human In Vitro System for Evaluating Antimycobacterial Vaccines. Infect. Immun. 72: 6401-6407 [Abstract] [Full Text]  
  • Lefevre, P., Peirs, P., Braibant, M., Fauville-Dufaux, M., Vanhoof, R., Huygen, K., Wang, X.-M., Pogell, B., Wang, Y., Fischer, P., Metz, P., Content, J. (2004). Antimycobacterial activity of synthetic pamamycins. J Antimicrob Chemother 54: 824-827 [Abstract] [Full Text]  
  • Verreck, F. A. W., de Boer, T., Langenberg, D. M. L., Hoeve, M. A., Kramer, M., Vaisberg, E., Kastelein, R., Kolk, A., de Waal-Malefyt, R., Ottenhoff, T. H. M. (2004). Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to (myco)bacteria. Proc. Natl. Acad. Sci. USA 101: 4560-4565 [Abstract] [Full Text]  
  • Huygen, K. (2003). On the Use of DNA Vaccines for the Prophylaxis of Mycobacterial Diseases. Infect. Immun. 71: 1613-1621 [Full Text]  
  • Middleton, A M, Chadwick, M V, Nicholson, A G, Dewar, A, Feldman, C, Wilson, R (2003). Investigation of mycobacterial colonisation and invasion of the respiratory mucosa. Thorax 58: 246-251 [Abstract] [Full Text]  
  • D'Souza, S., Rosseels, V., Romano, M., Tanghe, A., Denis, O., Jurion, F., Castiglione, N., Vanonckelen, A., Palfliet, K., Huygen, K. (2003). Mapping of Murine Th1 Helper T-Cell Epitopes of Mycolyl Transferases Ag85A, Ag85B, and Ag85C from Mycobacterium tuberculosis. Infect. Immun. 71: 483-493 [Abstract] [Full Text]  
  • Turner, D. J., Hoyle, S. L., Snewin, V. A., Gares, M.-P., Brown, I. N., Young, D. B. (2002). An ex vivo culture model for screening drug activity against in vivo phenotypes of Mycobacterium tuberculosis. Microbiology 148: 2929-2936 [Abstract] [Full Text]  
  • D'Souza, S., Rosseels, V., Denis, O., Tanghe, A., De Smet, N., Jurion, F., Palfliet, K., Castiglioni, N., Vanonckelen, A., Wheeler, C., Huygen, K. (2002). Improved Tuberculosis DNA Vaccines by Formulation in Cationic Lipids. Infect. Immun. 70: 3681-3688 [Abstract] [Full Text]  
  • Marshall, B. G., Wangoo, A., O'Gaora, P., Cook, H. T., Shaw, R. J., Young, D. B. (2001). Enhanced Antimycobacterial Response to Recombinant Mycobacterium bovis BCG Expressing Latency-Associated Peptide. Infect. Immun. 69: 6676-6682 [Abstract] [Full Text]  
  • Fairbairn, I. P., Stober, C. B., Kumararatne, D. S., Lammas, D. A. (2001). ATP-Mediated Killing of Intracellular Mycobacteria by Macrophages Is a P2X7-Dependent Process Inducing Bacterial Death by Phagosome-Lysosome Fusion. J. Immunol. 167: 3300-3307 [Abstract] [Full Text]  
  • Tanghe, A., D'Souza, S., Rosseels, V., Denis, O., Ottenhoff, T. H. M., Dalemans, W., Wheeler, C., Huygen, K. (2001). Improved Immunogenicity and Protective Efficacy of a Tuberculosis DNA Vaccine Encoding Ag85 by Protein Boosting. Infect. Immun. 69: 3041-3047 [Abstract] [Full Text]  
  • Neyrolles, O., Gould, K., Gares, M.-P., Brett, S., Janssen, R., O'Gaora, P., Herrmann, J.-L., Prevost, M.-C., Perret, E., Thole, J. E. R., Young, D. (2001). Lipoprotein Access to MHC Class I Presentation During Infection of Murine Macrophages with Live Mycobacteria. J. Immunol. 166: 447-457 [Abstract] [Full Text]  
  • Primm, T. P., Andersen, S. J., Mizrahi, V., Avarbock, D., Rubin, H., Barry, C. E. III (2000). The Stringent Response of Mycobacterium tuberculosis Is Required for Long-Term Survival. J. Bacteriol. 182: 4889-4898 [Abstract] [Full Text]  
  • De Voss, J. J., Rutter, K., Schroeder, B. G., Su, H., Zhu, Y., Barry, C. E. III (2000). The salicylate-derived mycobactin siderophores of Mycobacterium tuberculosis are essential for growth in macrophages. Proc. Natl. Acad. Sci. USA 97: 1252-1257 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»