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Infection and Immunity, September 1999, p. 4794-4800, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Porin from Pseudomonas aeruginosa Induces Apoptosis in an Epithelial Cell Line Derived from Rat Seminal Vesicles

Elisabetta Buommino,1,2 Francesco Morelli,1,3 Salvatore Metafora,1,3 Fabio Rossano,2 Brunella Perfetto,2 Adone Baroni,4 and Maria Antonietta Tufano2,*

CNR International Institute of Genetics and Biophysics,1 Servizio di Tecnologie Biomolecolari, Area di Ricerca del C.N.R.,3 and Istituto di Microbiologia,2 and Istituto di Clinica Dermosifilopatica,4 Medical School, 2nd University of Naples, Naples, Italy

Received 21 January 1999/Returned for modification 15 March 1999/Accepted 12 May 1999

Micromolar concentrations of porin, purified from the outer membranes of Pseudomonas aeruginosa, induced in vitro the classic morphological and biochemical signs of apoptosis in an epithelial cell line (SVC1) derived from the rat seminal vesicle secretory epithelium. The programmed cell death (PCD) was p53 independent and associated with significant decrease of bcl-2 expression, a marked increase of c-myc transcriptional activity, and an absence of the mRNA coding for tissue transglutaminase. The Ca2+ influx, caused by the porin treatment of SVC1 cells, appears to play an important role in the triggering of apoptosis in our biological model. The possibility that the porin property of inducing PCD plays a role in the infertility of individuals chronically infected by gram-negative bacteria is discussed.

* Corresponding author. Mailing address: Istituto di Microbiologia, Medical School, 2nd University of Naples, L.go S. Aniello a Caponapoli n. 2, 80138 Naples, Italy. Phone: 39 081-5665667. Fax: 39 081-5665663. E-mail: tufano{at}unina.it.

Infection and Immunity, September 1999, p. 4794-4800, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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