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Infection and Immunity, September 1999, p. 4902-4907, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Use of Genetically Manipulated Strains of Clostridium perfringens Reveals that Both Alpha-Toxin and Theta-Toxin Are Required for Vascular Leukostasis To Occur in Experimental Gas Gangrene

Darren M. Ellemor,1,* Rebecca N. Baird,1 Milena M. Awad,2 Richard L. Boyd,1 Julian I. Rood,2 and John J. Emmins1

Department of Pathology and Immunology, Monash Medical School, Alfred Hospital, Prahran, Victoria 3181,1 and Department of Microbiology, Monash University, Clayton, Victoria 3168,2 Australia

Received 24 February 1999/Returned for modification 26 April 1999/Accepted 15 June 1999

A hallmark of gas gangrene (clostridial myonecrosis) pathology is a paucity of leukocytes infiltrating the necrotic tissue. The cause of this paucity most likely relates to the observation of leukocyte aggregates at the border of the area of tissue necrosis, often within the microvasculature itself. Infecting mice with genetically manipulated strains of Clostridium perfringens type A (deficient in either alpha-toxin or theta-toxin production) resulted in significantly reduced leukocyte aggregation when alpha-toxin was absent and complete abrogation of leukocyte aggregation when theta-toxin was absent. Thus, both alpha-toxin and theta-toxin are necessary for the characteristic vascular leukostasis observed in clostridial myonecrosis.

* Corresponding author. Mailing address: Department of Pathology and Immunology, Monash University Medical School, Alfred Hospital, Prahran, Victoria 3181, Australia. Phone: 613 9903 0279. Fax: 613 9903 0731. E-mail: Darren.Ellemor{at}med.monash.edu.au.

Infection and Immunity, September 1999, p. 4902-4907, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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