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Infection and Immunity, September 1999, p. 4908-4911, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Adhesion of Escherichia coli to HeLa Cells Mediated by Trypanosoma cruzi Surface Glycoprotein-Derived Peptides Inserted in the Outer Membrane Protein LamB

Cátia M. Pereira, Sílvio Favoreto Jr., José Franco da Silveira, Nobuko Yoshida, and Beatriz A. Castilho*

Departamento de Microbiologia, Imunologia e Parasitologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, 04023-062, Brazil

Received 4 March 1999/Returned for modification 4 May 1999/Accepted 28 May 1999

Peptides derived from the surface glycoprotein gp82 of Trypanosoma cruzi, previously implicated in the parasite's invasion of host cells, were expressed as fusions to the protein LamB of Escherichia coli in a region known to be exposed on the cell surface. Bacteria expressing these proteins adhered to HeLa cells in a manner that mimics the pattern of parasite invasion of mammalian cells. Purified LamB fusion proteins were shown to bind to HeLa cells and to inhibit infection by T. cruzi, supporting the notion that these gp82-derived peptides can mediate interaction of the parasite with its host.

* Corresponding author. Mailing address: Disciplina de Microbiologia, Rua Botucatú, 862, 3° andar, São Paulo, SP, 04023-062, Brazil. Phone: (55)(11) 576-4537. Fax: (55)(11) 571-6504. E-mail: bac.dmip{at}epm.br.

Infection and Immunity, September 1999, p. 4908-4911, Vol. 67, No. 9
0019-9567/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Manque, P. M., Neira, I., Atayde, V. D., Cordero, E., Ferreira, A. T., da Silveira, J. F., Ramirez, M., Yoshida, N. (2003). Cell Adhesion and Ca2+ Signaling Activity in Stably Transfected Trypanosoma cruzi Epimastigotes Expressing the Metacyclic Stage-Specific Surface Molecule gp82. Infect. Immun. 71: 1561-1565 [Abstract] [Full Text]  
  • Manque, P. M., Eichinger, D., Juliano, M. A., Juliano, L., Araya, J. E., Yoshida, N. (2000). Characterization of the Cell Adhesion Site of Trypanosoma cruzi Metacyclic Stage Surface Glycoprotein gp82. Infect. Immun. 68: 478-484 [Abstract] [Full Text]  


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