This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by García-Ojeda, P. A.
Right arrow Articles by Stein, K. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by García-Ojeda, P. A.
Right arrow Articles by Stein, K. E.

 Previous Article  |  Next Article 

Infection and Immunity, January 2000, p. 239-246, Vol. 68, No. 1
0019-9567/0/$04.00+0

Murine Immune Response to Neisseria meningitidis Group C Capsular Polysaccharide: Analysis of Monoclonal Antibodies Generated in Response to a Thymus-Independent Antigen and a Thymus-Dependent Toxoid Conjugate Vaccine

Pablo A. García-Ojeda,1 Martha E. Monser,1,dagger Leonard J. Rubinstein,1,Dagger Harold J. Jennings,2 and Kathryn E. Stein1,*

Division of Monoclonal Antibodies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892,1 and Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A OR62

Received 21 July 1999/Accepted 20 October 1999

Antibody (Ab) responses to polysaccharides (PSs) such as Neisseria meningitidis group C PS (MCPS) are characterized as being thymus independent (TI) and are restricted with regard to clonotype and isotype expression. PS conjugated to proteins, e.g., MCPS coupled to tetanus toxoid (MCPS-TT), elicits a thymus-dependent (TD) response. In order to understand the influence of the form of a vaccine (TI versus TD) on the Ab repertoire, we generated monoclonal antibody (MAb) panels from mice immunized and boosted with MCPS or MCPS-TT in different ways. The panels of MAbs were examined for isotype, fine specificity, affinity, and VH gene family usage. The use of MCPS-TT resulted in a shift in the isotype from immunoglobulin M (IgM) and IgG3 elicited in response to the MCPS to primarily IgG1. This isotype shift was accompanied by a change in the fine specificity of the response to the conjugate compared to that of PS. New fine specificities and increased affinity were observed in response to the TD antigen (Ag). Dot blot and Northern analyses of MCPS MAbs revealed that VH gene family usage is dominated by VHJ558, used by 23 of 39 MAbs. VH3609 was seen in three MAbs of restricted fine specificity. VHQ52, VH7183, and VHVGAM3-8 were seen in more than one MAb across these panels, while VH10 and VHX24 were detected only once in response to the TI-2 Ag. All MAbs in the panels utilized kappa light chains, and all functional Jkappa genes were expressed.

* Corresponding author. Mailing address: Division of Monoclonal Antibodies, CBER, FDA, 29 Lincoln Dr., Bethesda, MD 20892. Phone: (301) 827-0850. Fax: (301) 827-0852. E-mail: stein{at}cber.fda.gov.

dagger Present address: Division of Vaccines and Related Products Applications, CBER, FDA, Rockville, MD 20852.

Dagger Present address: Developmental Human Vaccine Serology, Merck Research Laboratories, West Point, PA 19486.

Infection and Immunity, January 2000, p. 239-246, Vol. 68, No. 1
0019-9567/0/$04.00+0


This article has been cited by other articles:

  • Tian, H., Groner, A., Boes, M., Pirofski, L.-a. (2007). Pneumococcal Capsular Polysaccharide Vaccine-Mediated Protection against Serotype 3 Streptococcus pneumoniae in Immunodeficient Mice. Infect. Immun. 75: 1643-1650 [Abstract] [Full Text]  
  • Harris, S. L., Tsao, H., Ashton, L., Goldblatt, D., Fernsten, P. (2007). Avidity of the Immunoglobulin G Response to a Neisseria meningitidis Group C Polysaccharide Conjugate Vaccine as Measured by Inhibition and Chaotropic Enzyme-Linked Immunosorbent Assays. CVI 14: 397-403 [Abstract] [Full Text]  
  • Goel, M., Krishnan, L., Kaur, S., Kaur, K. J., Salunke, D. M. (2004). Plasticity within the Antigen-Combining Site May Manifest as Molecular Mimicry in the Humoral Immune Response. J. Immunol. 173: 7358-7367 [Abstract] [Full Text]  
  • Garcia-Ojeda, P. A., Hardy, S., Kozlowski, S., Stein, K. E., Feavers, I. M. (2004). Surface Plasmon Resonance Analysis of Antipolysaccharide Antibody Specificity: Responses to Meningococcal Group C Conjugate Vaccines and Bacteria. Infect. Immun. 72: 3451-3460 [Abstract] [Full Text]  
  • Welsch, J. A., Rossi, R., Comanducci, M., Granoff, D. M. (2004). Protective Activity of Monoclonal Antibodies to Genome-Derived Neisserial Antigen 1870, a Neisseria meningitidis Candidate Vaccine. J. Immunol. 172: 5606-5615 [Abstract] [Full Text]  
  • Harris, S. L., King, W. J., Ferris, W., Granoff, D. M. (2003). Age-Related Disparity in Functional Activities of Human Group C Serum Anticapsular Antibodies Elicited by Meningococcal Polysaccharide Vaccine. Infect. Immun. 71: 275-286 [Abstract] [Full Text]  
  • Moe, G. R., Zuno-Mitchell, P., Hammond, S. N., Granoff, D. M. (2002). Sequential Immunization with Vesicles Prepared from Heterologous Neisseria meningitidis Strains Elicits Broadly Protective Serum Antibodies to Group B Strains. Infect. Immun. 70: 6021-6031 [Abstract] [Full Text]  
  • Granoff, D. M., Moe, G. R., Giuliani, M. M., Adu-Bobie, J., Santini, L., Brunelli, B., Piccinetti, F., Zuno-Mitchell, P., Lee, S. S., Neri, P., Bracci, L., Lozzi, L., Rappuoli, R. (2001). A Novel Mimetic Antigen Eliciting Protective Antibody to Neisseria meningitidis. J. Immunol. 167: 6487-6496 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»