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Infection and Immunity, January 2000, p. 377-381, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A P5 Peptide That Is Homologous to Peptide 10 of OprF from Pseudomonas aeruginosa Enhances Clearance of Nontypeable Haemophilus influenzae from Acutely Infected Rat Lung in the Absence of Detectable Peptide-Specific Antibody

Dianne C. Webb1,2,* and Allan W. Cripps1

The Gadi Research Center, Faculty of Applied Science and Design, University of Canberra,1 and The Membrane Biochemistry Group, Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University,2 Canberra City, Australian Capital Territory 2601, Australia

Received 23 August 1999/Returned for modification 29 September 1999/Accepted 22 October 1999

Nontypeable Haemophilus influenzae (NTHi) is an opportunistic pathogen associated with otitis media and the exacerbation of chronic bronchitis. This study reports the vaccine potential of three peptides representing conserved regions of the NTHi P5 outer membrane protein which have been fused to a promiscuous measles virus F protein T-cell eptitope (MVF). The peptides correspond to a region in surface loop one (MVF/L1A), the central region of loop four (MVF/L4), and a C-terminal region homologous to peptide 10 of OprF from Pseudomonas aeruginosa (MVF/H3). Immunization of rats with MVF/H3 was the most efficacious in significantly reducing the number of viable NTHi in both the broncho-alveolar lavage fluid (74%) and lung homogenates (70%), compared to control rats. Importantly, despite significantly increased rates of clearance, immunization with MVF/H3 elicited poor antibody responses, suggesting that cell-mediated rather than humoral responses play an important role in the enhanced clearance of NTHi in this model.

* Corresponding author. Present address: Leukocyte Signaling and Regulation Laboratory, John Curtin School of Medical Research, Australian National University, P.O. Box 334, Canberra City, ACT 2601, Australia. Phone: (61) 6 249 5471. Fax: (61) 6 249 0415. E-mail: Dianne.Webb{at}anu.edu.au.

Infection and Immunity, January 2000, p. 377-381, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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