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Infection and Immunity, January 2000, p. 64-71, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Typing of Intimin Genes in Human and Animal Enterohemorrhagic and
Enteropathogenic Escherichia coli: Characterization of a
New Intimin Variant
E.
Oswald,1,*
H.
Schmidt,2
S.
Morabito,3
H.
Karch,2
O.
Marchès,1 and
A.
Caprioli3
Unité INRA-ENVT de Microbiologie
Moléculaire, Ecole Vétérinaire de Toulouse, 31076 Toulouse Cedex, France1; Institut
für Hygiene und Mikrobiologie, University of Wurzburg, Wurzburg
97080, Germany2; and Laboratorio di
Medicina Veterinaria, Istituto Superiore di Sanità, 00161 Rome,
Italy3
Received 26 July 1999/Returned for modification 8 September
1999/Accepted 30 September 1999
Enteropathogenic Escherichia coli (EPEC) and
enterohemorrhagic E. coli (EHEC) produce the characteristic
"attaching and effacing" (A/E) lesion of the brush border. Intimin,
an outer membrane protein encoded by eae, is responsible
for the tight association of both pathogens with the host cell. Several
eae have been cloned from different EPEC and EHEC strains
isolated from humans and animals. These sequences are conserved in the
N-terminal region but highly variable in the last C-terminal 280 amino
acids (aa), where the cell binding activity is localized. Based on
these considerations, we developed a panel of specific primers to
investigate the eae heterogeneity of the variable 3' region
by using PCR amplification. We then investigated the distribution of
the known intimin types in a large collection of EPEC and EHEC strains
isolated from humans and different animal species. The existence of a
yet-unknown family of intimin was suspected because several EHEC
strains, isolated from human and cattle, did not react with any of the
specific primer pairs, although these strains were eae
positive when primers amplifying the conserved 5' end were used. We
then cloned and sequenced the eae present in one of these
strains (EHEC of serotype O103:H2) and subsequently designed a PCR
primer that recognizes in a specific manner the variable 3' region of
this new intimin type. This intimin, referred to as "
," was
present in human and bovine EHEC strains of serogroups O8, O11, O45,
O103, O121, and O165. Intimin
is the largest intimin cloned to date
(948 aa) and shares the greatest overall sequence identity with intimin
, although analysis of the last C-terminal 280 aa suggests a greater
similarity with intimins
and
.
*
Corresponding author. Mailing address: Unité
mixte INRA-ENVT de Microbiologie Moléculaire, 23 chemin des
Capelles, 31076 Toulouse Cedex, France. Phone: (33) 561-19-39-91. Fax:
(33) 561-19-39-75. E-mail: e.oswald{at}envt.fr.
Infection and Immunity, January 2000, p. 64-71, Vol. 68, No. 1
0019-9567/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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[Full Text]
-
Stevens, M. P., Marches, O., Campbell, J., Huter, V., Frankel, G., Phillips, A. D., Oswald, E., Wallis, T. S.
(2002). Intimin, Tir, and Shiga Toxin 1 Do Not Influence Enteropathogenic Responses to Shiga Toxin-Producing Escherichia coli in Bovine Ligated Intestinal Loops. Infect. Immun.
70: 945-952
[Abstract]
[Full Text]
-
Fitzhenry, R J, Pickard, D J, Hartland, E L, Reece, S, Dougan, G, Phillips, A D, Frankel, G
(2002). Intimin type influences the site of human intestinal mucosal colonisation by enterohaemorrhagic Escherichia coli O157:H7. Gut
50: 180-185
[Abstract]
[Full Text]
-
Son, W.-G., Graham, T. A., Gannon, V. P. J.
(2002). Immunological Characterization of Escherichia coli O157:H7 Intimin {gamma}1. CVI
9: 46-53
[Abstract]
[Full Text]
-
Wentzel, A., Christmann, A., Adams, T., Kolmar, H.
(2001). Display of Passenger Proteins on the Surface of Escherichia coli K-12 by the Enterohemorrhagic E. coli Intimin EaeA. J. Bacteriol.
183: 7273-7284
[Abstract]
[Full Text]
-
Deng, W., Li, Y., Vallance, B. A., Finlay, B. B.
(2001). Locus of Enterocyte Effacement from Citrobacter rodentium: Sequence Analysis and Evidence for Horizontal Transfer among Attaching and Effacing Pathogens. Infect. Immun.
69: 6323-6335
[Abstract]
[Full Text]
-
Ghaem-Maghami, M., Simmons, C. P., Daniell, S., Pizza, M., Lewis, D., Frankel, G., Dougan, G.
(2001). Intimin-Specific Immune Responses Prevent Bacterial Colonization by the Attaching-Effacing Pathogen Citrobacter rodentium. Infect. Immun.
69: 5597-5605
[Abstract]
[Full Text]
-
Cid, D., Ruiz-Santa-Quiteria, J. A., Marin, I., Sanz, R., Orden, J. A., Amils, R., de la Fuente, R.
(2001). Association between intimin (eae) and EspB gene subtypes in attaching and effacing Escherichia coli strains isolated from diarrhoeic lambs and goat kids. Microbiology
147: 2341-2353
[Abstract]
[Full Text]
-
Karch, H., Bielaszewska, M.
(2001). Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H{-} Strains: Epidemiology, Phenotypic and Molecular Characteristics, and Microbiological Diagnosis. J. Clin. Microbiol.
39: 2043-2049
[Full Text]
-
Zhu, C., Agin, T. S., Elliott, S. J., Johnson, L. A., Thate, T. E., Kaper, J. B., Boedeker, E. C.
(2001). Complete Nucleotide Sequence and Analysis of the Locus of Enterocyte Effacement from Rabbit Diarrheagenic Escherichia coli RDEC-1. Infect. Immun.
69: 2107-2115
[Abstract]
[Full Text]
-
Mansfield, K. G., Lin, K.-C., Newman, J., Schauer, D., MacKey, J., Lackner, A. A., Carville, A.
(2001). Identification of Enteropathogenic Escherichia coli in Simian Immunodeficiency Virus-Infected Infant and Adult Rhesus Macaques. J. Clin. Microbiol.
39: 971-976
[Abstract]
[Full Text]
-
Kobayashi, H., Shimada, J., Nakazawa, M., Morozumi, T., Pohjanvirta, T., Pelkonen, S., Yamamoto, K.
(2001). Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan. Appl. Environ. Microbiol.
67: 484-489
[Abstract]
[Full Text]
-
Bielaszewska, M., Schmidt, H., Liesegang, A., Prager, R., Rabsch, W., Tschäpe, H., Cízek, A., Janda, J., Bláhová, K., Karch, H.
(2000). Cattle Can Be a Reservoir of Sorbitol-Fermenting Shiga Toxin-Producing Escherichia coli O157:H- Strains and a Source of Human Diseases. J. Clin. Microbiol.
38: 3470-3473
[Abstract]
[Full Text]
-
Hartland, E. L., Huter, V., Higgins, L. M., Goncalves, N. S., Dougan, G., Phillips, A. D., MacDonald, T. T., Frankel, G.
(2000). Expression of Intimin gamma from Enterohemorrhagic Escherichia coli in Citrobacter rodentium. Infect. Immun.
68: 4637-4646
[Abstract]
[Full Text]
-
Zhang, W.-L., Bielaszewska, M., Liesegang, A., Tschäpe, H., Schmidt, H., Bitzan, M., Karch, H.
(2000). Molecular Characteristics and Epidemiological Significance of Shiga Toxin-Producing Escherichia coli O26 Strains. J. Clin. Microbiol.
38: 2134-2140
[Abstract]
[Full Text]
-
Sinclair, J. F., O'Brien, A. D.
(2002). Cell Surface-localized Nucleolin Is a Eukaryotic Receptor for the Adhesin Intimin-gamma of Enterohemorrhagic Escherichia coli O157:H7. J. Biol. Chem.
277: 2876-2885
[Abstract]
[Full Text]