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Infection and Immunity, October 2000, p. 5539-5545, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Generation of Female Genital Tract Antibody Responses by Local or Central (Common) Mucosal Immunization

Hong-Yin Wu,dagger Samira Abdu, Dana Stinson, and Michael W. Russell*

Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama

Received 17 April 2000/Returned for modification 19 June 2000/Accepted 3 July 2000

Genital antibody responses were compared in female mice immunized intravaginally (i.vag.) or intranasally (i.n.) with a bacterial protein antigen (AgI/II of Streptococcus mutans) coupled to the B subunit of cholera toxin. Serum and salivary antibodies were also evaluated as measures of disseminated mucosal and systemic responses. Although i.vag. immunization induced local vaginal immunoglobulin A (IgA) and IgG antibody responses, these were not disseminated to a remote secretion, the saliva, and only modest levels of serum antibodies were generated. In contrast, i.n. immunization was substantially more effective at inducing IgA and IgG antibody responses in the genital tract and in the circulation, as well as at inducing IgA antibodies in the saliva. Moreover, mucosal and systemic antibodies induced by i.n. immunization persisted for at least 12 months. Analysis of the molecular form of genital IgA indicated that the majority of both total IgA and specific IgA antibody was polymeric, and likely derived from the common mucosal immune system.

* Corresponding author. Mailing address: Department of Microbiology, University of Alabama at Birmingham, 845, 19th St. South, Birmingham, AL 35294-2170. Phone: (205) 934-4480. Fax: (205) 934-3894. E-mail: MWR{at}uab.edu.

dagger Present address: Wyeth-Lederle Vaccines and Pediatrics, Pearl River, NY 10965.

Infection and Immunity, October 2000, p. 5539-5545, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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