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Infection and Immunity, October 2000, p. 5559-5566, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Analysis of Human Antibodies to Erythrocyte Binding
Antigen 175 of Plasmodium falciparum
Daniel M. N.
Okenu,1,2
Eleanor M.
Riley,1
Quentin D.
Bickle,1
Philip U.
Agomo,2
Arnoldo
Barbosa,3
Jon R.
Daugherty,3
David E.
Lanar,3 and
David J.
Conway1,*
Department of Infectious and Tropical
Diseases, London School of Hygiene and Tropical Medicine, London WC1E
7HT, United Kingdom1; Division of
Biochemistry, National Institute for Medical Research, Lagos,
Nigeria2; and Department of Immunology,
Walter Reed Army Institute of Research, Silver Spring, Maryland
209103
Received 22 May 2000/Returned for modification 10 June
2000/Accepted 4 July 2000
Invasion of human erythrocytes by Plasmodium falciparum
merozoites is a multistep process. For many strains of the parasite, part of this process requires that the erythrocyte binding antigen 175 (EBA-175) of the merozoite binds to sialic acid residues of glycophorin
A on the erythrocyte surface, a receptor-ligand interaction which
represents a potential target for inhibition by antibodies. This study
characterizes the reactivity of naturally acquired human antibodies
with four recombinant proteins representing parts of EBA-175 (region
II, regions III to V, and the dimorphic C and F segment region) in
populations in which the organism is endemic. Serum immunoglobulin G
(IgG) recognizing the recombinant proteins is predominantly of the IgG1
and IgG3 subclasses, and its prevalence increases with age. In a large
population study in The Gambia, serum positivity for IgG or IgG1 and
IgG3 subclass antibodies to each of the EBA-175 recombinant antigens
was not significantly associated with subsequent protection from
clinical malaria. However, there was a trend indicating that
individuals with high levels of IgG to region II may have some protection.
*
Corresponding author. Mailing address: Department of
Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St., London WC1E 7HT, United Kingdom. Phone: 44-20-7927-2331. Fax: 44-20-7636 8739. E-mail:
david.conway{at}lshtm.ac.uk.
Infection and Immunity, October 2000, p. 5559-5566, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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