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Infection and Immunity, October 2000, p. 5559-5566, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Analysis of Human Antibodies to Erythrocyte Binding Antigen 175 of Plasmodium falciparum

Daniel M. N. Okenu,1,2 Eleanor M. Riley,1 Quentin D. Bickle,1 Philip U. Agomo,2 Arnoldo Barbosa,3 Jon R. Daugherty,3 David E. Lanar,3 and David J. Conway1,*

Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom1; Division of Biochemistry, National Institute for Medical Research, Lagos, Nigeria2; and Department of Immunology, Walter Reed Army Institute of Research, Silver Spring, Maryland 209103

Received 22 May 2000/Returned for modification 10 June 2000/Accepted 4 July 2000

Invasion of human erythrocytes by Plasmodium falciparum merozoites is a multistep process. For many strains of the parasite, part of this process requires that the erythrocyte binding antigen 175 (EBA-175) of the merozoite binds to sialic acid residues of glycophorin A on the erythrocyte surface, a receptor-ligand interaction which represents a potential target for inhibition by antibodies. This study characterizes the reactivity of naturally acquired human antibodies with four recombinant proteins representing parts of EBA-175 (region II, regions III to V, and the dimorphic C and F segment region) in populations in which the organism is endemic. Serum immunoglobulin G (IgG) recognizing the recombinant proteins is predominantly of the IgG1 and IgG3 subclasses, and its prevalence increases with age. In a large population study in The Gambia, serum positivity for IgG or IgG1 and IgG3 subclass antibodies to each of the EBA-175 recombinant antigens was not significantly associated with subsequent protection from clinical malaria. However, there was a trend indicating that individuals with high levels of IgG to region II may have some protection.

* Corresponding author. Mailing address: Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St., London WC1E 7HT, United Kingdom. Phone: 44-20-7927-2331. Fax: 44-20-7636 8739. E-mail: david.conway{at}lshtm.ac.uk.

Infection and Immunity, October 2000, p. 5559-5566, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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