This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by M, A.
Right arrow Articles by Marchal, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by M, A.
Right arrow Articles by Marchal, G.

 Previous Article  |  Next Article 

Infection and Immunity, October 2000, p. 5657-5662, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mycobacterium bovis BCG Induces Similar Immune Responses and Protection by Rectal and Parenteral Immunization Routes

Abolhassani M,1,2 M. Lagranderie,1 P. Chavarot,3 A.-M. Balazuc,1 and G. Marchal1,3,*

Laboratoire du BCG1 and Unité de Physiopathologie de l'Infection,3 Institut Pasteur, 75724 Paris Cedex 15, France, and Département du BCG, Institut Pasteur de Téhéran, Teheran, Iran2

Received 31 March 2000/Returned for modification 5 May 2000/Accepted 12 July 2000

We compared cellular immune responses to rectal, subcutaneous, and intradermal administration of Mycobacterium bovis BCG for 5 to 20 weeks in mice, guinea pigs, and macaques. Strong lymphoproliferative responses were induced in spleen cells after in vitro stimulation with purified protein derivative in guinea pigs and macaques, whatever the route of immunization. Comparable high numbers of gamma interferon- and tumor necrosis factor alpha-producing cells were found in the spleen after rectal, subcutaneous, and intradermal immunization of mice and macaques. Similar levels of precursors of cytotoxic T lymphocytes specific for mycobacterial antigens were observed in mice for all immunization routes. In macaques, cytotoxic activity, determined only at the end of the experiment (20 weeks), was similar after rectal and intradermal immunization. Six months after immunization, rectal and subcutaneous routes induced in mice similar levels of protective immunity against challenge with a virulent Mycobacterium tuberculosis strain (H37Rv). Rectal immunization gave immune responses and protective capacity similar to those for parenteral immunization and seemed to be a promising new route of vaccination against tuberculosis; in our study, immunization via the rectal route never induced side effects associated with parenteral routes (axillary adenitis) and could also effectively reduce the risks of viral transmission associated with unsafe injections in the developing world.

* Corresponding author. Mailing address: Unité de Physiopathologie, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: (33) 1 45 68 86 68. Fax: (33) 1 40 61 33 32. E-mail: gmarchal{at}pasteur.fr.

Infection and Immunity, October 2000, p. 5657-5662, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Abolhassani, M., Guais, A., Chaumet-Riffaud, P., Sasco, A. J., Schwartz, L. (2009). Carbon dioxide inhalation causes pulmonary inflammation. Am. J. Physiol. Lung Cell. Mol. Physiol. 296: L657-L665 [Abstract] [Full Text]  
  • Mittrucker, H.-W., Steinhoff, U., Kohler, A., Krause, M., Lazar, D., Mex, P., Miekley, D., Kaufmann, S. H. E. (2007). Poor correlation between BCG vaccination-induced T cell responses and protection against tuberculosis. Proc. Natl. Acad. Sci. USA 104: 12434-12439 [Abstract] [Full Text]  
  • Castillo-Rodal, A. I., Castanon-Arreola, M., Hernandez-Pando, R., Calva, J. J., Sada-Diaz, E., Lopez-Vidal, Y. (2006). Mycobacterium bovis BCG Substrains Confer Different Levels of Protection against Mycobacterium tuberculosis Infection in a BALB/c Model of Progressive Pulmonary Tuberculosis. Infect. Immun. 74: 1718-1724 [Abstract] [Full Text]  
  • Parez, N., Fourgeux, C., Mohamed, A., Dubuquoy, C., Pillot, M., Dehee, A., Charpilienne, A., Poncet, D., Schwartz-Cornil, I., Garbarg-Chenon, A. (2006). Rectal Immunization with Rotavirus Virus-Like Particles Induces Systemic and Mucosal Humoral Immune Responses and Protects Mice against Rotavirus Infection. J. Virol. 80: 1752-1761 [Abstract] [Full Text]  
  • Chen, L., Wang, J., Zganiacz, A., Xing, Z. (2004). Single Intranasal Mucosal Mycobacterium bovis BCG Vaccination Confers Improved Protection Compared to Subcutaneous Vaccination against Pulmonary Tuberculosis. Infect. Immun. 72: 238-246 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»