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Infection and Immunity, October 2000, p. 5908-5913, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Modulation of Neutrophil Chemokine Receptors by Staphylococcus aureus Supernate

K. E. Veldkamp,* H. C. J. M. Heezius, J. Verhoef, J. A. G. van Strijp, and K. P. M. van Kessel

Eijkman-Winkler Institute, Utrecht University, Utrecht, The Netherlands

Received 20 March 2000/Returned for modification 15 May 2000/Accepted 16 July 2000

In a previous study, we showed that Staphylococcus aureus supernate (SaS) is a potent agonist for both neutrophils and mononuclear cells. To further investigate the immunomodulating effects of SaS, the effect on different neutrophil receptors was studied. Expression of various neutrophil receptors, before and after treatment with SaS, was quantified by flow cytometry. We found that SaS treatment of neutrophils resulted in a specific and total downregulation of the C5a and the fMLP receptor, both serpentine receptors, while other receptors were totally unaffected. Since these two receptors are both involved in chemotaxis, we tested the effect of SaS in calcium flux and chemotaxis assays. We showed that preincubation with SaS abrogated the rise in intracellular calcium concentration upon triggering with fMLP and C5a. We also showed that SaS is a potent inhibitor of neutrophil chemotaxis towards fMLP and C5a, but does not interfere with chemotaxis towards interleukin-8. These findings indicate that S. aureus produces a virulence factor extracellularly, which impairs chemotaxis towards the infected site.

* Corresponding author: Mailing address: Eijkman-Winkler Institute, University of Utrecht, G04.612, Heidelberglaan 100, 3508 GA Utrecht, The Netherlands. Phone: 31 30 2506528. Fax: 31 30 2541770. E-mail: K.E.Veldkamp{at}lab.azu.nl.

Infection and Immunity, October 2000, p. 5908-5913, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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