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Infection and Immunity, October 2000, p. 6082-6086, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Peritoneal Culture Alters Streptococcus pneumoniae Protein Profiles and Virulence Properties

Carlos J. Orihuela,1 Rob Janssen,2 Christopher W. Robb,1 David A. Watson,1,3 and David W. Niesel1,*

Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas 77555-10191; Eijkman-Winkler Institute for Microbiology, Infection Diseases and Inflammation, Utrecht University, 3584 CX Utrecht, The Netherlands2; and InDyne, Inc., Nassau Bay, Texas 770583

Received 17 April 2000/Returned for modification 21 June 2000/Accepted 3 July 2000

We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555-1019. Phone: (409) 747-6842. Fax: (409) 747-6869. E-mail: dniesel{at}utmb.edu.

Infection and Immunity, October 2000, p. 6082-6086, Vol. 68, No. 10
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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