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Infection and Immunity, November 2000, p. 6487-6492, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Association of Protease Activity in Vibrio cholerae Vaccine Strains with Decreases in Transcellular Epithelial Resistance of Polarized T84 Intestinal Epithelial Cells

Stephanie F. Mel,1,dagger Karla Jean Fullner,1 Susan Wimer-Mackin,2 Wayne I. Lencer,2 and John J. Mekalanos1,*

Department of Microbiology and Molecular Genetics, Harvard Medical School,1 and Combined Program in Pediatric Gastroenterology and Nutrition and Harvard Digestive Diseases Center,2 Boston, Massachusetts

Received 25 April 2000/Accepted 13 July 2000

Culture supernatants prepared from reactogenic strains of Vibrio cholerae cause a decrease in the transcellular epithelial resistance of T84 intestinal cells. This decrease correlates with the presence of hemagglutinin/protease but not with the presence of other potential accessory toxins or proteases. These data suggest a possible role for hemagglutinin/protease in reactogenicity, although other factors may also contribute.

* Corresponding author. Mailing address: Department of Microbiology and Molecular Genetics, 200 Longwood Ave. D1-420, Harvard Medical School, Boston, MA 02115. Phone: (617) 432-1935. Fax: (617) 738-7664. E-mail: jmekalanos{at}hms.harvard.edu.

dagger Present address: Department of Biology, University of California, San Diego, La Jolla, CA 92093.

Infection and Immunity, November 2000, p. 6487-6492, Vol. 68, No. 11
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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