This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lapinet, J. A.
Right arrow Articles by Cassatella, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lapinet, J. A.
Right arrow Articles by Cassatella, M. A.

 Previous Article  |  Next Article 

Infection and Immunity, December 2000, p. 6917-6923, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Gene Expression and Production of Tumor Necrosis Factor Alpha, Interleukin-1beta (IL-1beta ), IL-8, Macrophage Inflammatory Protein 1alpha (MIP-1alpha ), MIP-1beta , and Gamma Interferon-Inducible Protein 10 by Human Neutrophils Stimulated with Group B Meningococcal Outer Membrane Vesicles

José A. Lapinet,1,2 Patrizia Scapini,1 Federica Calzetti,1 Oliver Pérez,2 and Marco A. Cassatella1,*

Department of Pathology, Section of General Pathology, University of Verona, 37134 Verona, Italy,1 and Finlay Institute, 11600, Havana, Cuba2

Received 26 July 2000/Accepted 8 September 2000

Accumulation of polymorphonuclear neutrophils (PMN) into the subarachnoidal space is one of the hallmarks of Neisseria meningitidis infection. In this study, we evaluated the ability of outer membrane vesicles (OMV) from N. meningitidis B to stimulate cytokine production by neutrophils. We found that PMN stimulated in vitro by OMV produce proinflammatory cytokines and chemokines including tumor necrosis factor alpha (TNF-alpha ), interleukin-1beta (IL-1beta ), IL-8, macrophage inflammatory protein 1alpha (MIP-1alpha ), and MIP-1beta . A considerable induction of gamma interferon (IFN-gamma )-inducible protein 10 (IP-10) mRNA transcripts, as well as extracellular IP-10 release, was also observed when neutrophils were stimulated by OMV in combination with IFN-gamma . Furthermore, PMN stimulated by OMV in the presence of IFN-gamma demonstrated an enhanced capacity to release TNF-alpha , IL-1beta , IL-8, and MIP-1beta compared to stimulation with OMV alone. In line with its downregulatory effects on neutrophil-derived proinflammatory cytokines, IL-10 potently inhibited TNF-alpha , IL-1beta , IL-8, and MIP-1beta production triggered by OMV. Finally, a neutralizing anti-TNF-alpha monoclonal antibody (MAb) did not influence the release of IL-8 and MIP-1beta induced by OMV, therefore excluding a role for endogenous TNF-alpha in mediating the induction of chemokine release by OMV. In contrast, the ability of lipopolysaccharide from N. meningitidis B to induce the production of IL-8 and MIP-1beta was significantly inhibited by anti-TNF-alpha MAb. Our results establish that, in response to OMV, neutrophils produce a proinflammatory profile of cytokines and chemokines which may not only play a role in the pathogenesis of meningitis but may also contribute to the development of protective immunity to serogroup B meningococci.

* Corresponding author. Mailing address: Department of Pathology, Section of General Pathology, Strada Le Grazie 4, I-37134 Verona, Italy. Phone: 39-045-8027130. Fax: 39-045-8027127. E-mail: MCNCSS{at}borgoroma.univr.it.

Infection and Immunity, December 2000, p. 6917-6923, Vol. 68, No. 12
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tavano, R., Franzoso, S., Cecchini, P., Cartocci, E., Oriente, F., Arico, B., Papini, E. (2009). The membrane expression of Neisseria meningitidis adhesin A (NadA) increases the proimmune effects of MenB OMVs on human macrophages, compared with NadA- OMVs, without further stimulating their proinflammatory activity on circulating monocytes. J. Leukoc. Biol. 86: 143-153 [Abstract] [Full Text]  
  • Hurtado-Nedelec, M., Chollet-Martin, S., Nicaise-Roland, P., Grootenboer-Mignot, S., Ruimy, R., Meyer, O., Hayem, G. (2008). Characterization of the immune response in the synovitis, acne, pustulosis, hyperostosis, osteitis (SAPHO) syndrome. Rheumatology (Oxford) 47: 1160-1167 [Abstract] [Full Text]  
  • Polenghi, A., Bossi, F., Fischetti, F., Durigutto, P., Cabrelle, A., Tamassia, N., Cassatella, M. A., Montecucco, C., Tedesco, F., de Bernard, M. (2007). The Neutrophil-Activating Protein of Helicobacter pylori Crosses Endothelia to Promote Neutrophil Adhesion In Vivo. J. Immunol. 178: 1312-1320 [Abstract] [Full Text]  
  • Tavares, E., Minano, F. J., Maldonado, R., Dascombe, M. J. (2006). Endotoxin fever in granulocytopenic rats: evidence that brain cyclooxygenase-2 is more important than circulating prostaglandin E2. J. Leukoc. Biol. 80: 1375-1387 [Abstract] [Full Text]  
  • Plant, L., Wan, H., Jonsson, A.-B. (2006). MyD88-Dependent Signaling Affects the Development of Meningococcal Sepsis by Nonlipooligosaccharide Ligands.. Infect. Immun. 74: 3538-3546 [Abstract] [Full Text]  
  • Gordon, J. R., Li, F., Zhang, X., Wang, W., Zhao, X., Nayyar, A. (2005). The combined CXCR1/CXCR2 antagonist CXCL8(3-74)K11R/G31P blocks neutrophil infiltration, pyrexia, and pulmonary vascular pathology in endotoxemic animals. J. Leukoc. Biol. 78: 1265-1272 [Abstract] [Full Text]  
  • Krueger, G., Callis, K. (2004). Potential of Tumor Necrosis Factor Inhibitors in Psoriasis and Psoriatic Arthritis. Arch Dermatol 140: 218-225 [Abstract] [Full Text]  
  • von Stebut, E., Metz, M., Milon, G., Knop, J., Maurer, M. (2003). Early macrophage influx to sites of cutaneous granuloma formation is dependent on MIP-1alpha /beta released from neutrophils recruited by mast cell-derived TNFalpha. Blood 101: 210-215 [Abstract] [Full Text]  
  • Al-Mohanna, F., Saleh, S., Parhar, R. S., Collison, K. (2002). IL-12-dependent nuclear factor-{kappa}B activation leads to de novo synthesis and release of IL-8 and TNF-{alpha} in human neutrophils. J. Leukoc. Biol. 72: 995-1002 [Abstract] [Full Text]  
  • Crepaldi, L., Silveri, L., Calzetti, F., Pinardi, C., Cassatella, M. A. (2002). Molecular basis of the synergistic production of IL-1 receptor antagonist by human neutrophils stimulated with IL-4 and IL-10. Int Immunol 14: 1145-1153 [Abstract] [Full Text]  
  • Khan, A. Q., Shen, Y., Wu, Z.-Q., Wynn, T. A., Snapper, C. M. (2002). Endogenous Pro- and Anti-Inflammatory Cytokines Differentially Regulate an In Vivo Humoral Response to Streptococcus pneumoniae. Infect. Immun. 70: 749-761 [Abstract] [Full Text]  
  • Brandtzaeg, P., Bjerre, A., Ovstebo, R., Brusletto, B., Joo, G. B., Kierulf, P. (2001). Invited review: Neisseria meningitidis lipopolysaccharides in human pathology. Innate Immunity 7: 401-420 [Abstract]  
  • Kolb-Maurer, A., Unkmeir, A., Kammerer, U., Hubner, C., Leimbach, T., Stade, A., Kampgen, E., Frosch, M., Dietrich, G. (2001). Interaction of Neisseria meningitidis with Human Dendritic Cells. Infect. Immun. 69: 6912-6922 [Abstract] [Full Text]  
  • Crepaldi, L., Gasperini, S., Lapinet, J. A., Calzetti, F., Pinardi, C., Liu, Y., Zurawski, S., de Waal Malefyt, R., Moore, K. W., Cassatella, M. A. (2001). Up-Regulation of IL-10R1 Expression Is Required to Render Human Neutrophils Fully Responsive to IL-10. J. Immunol. 167: 2312-2322 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»