This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tong, H. H.
Right arrow Articles by DeMaria, T. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tong, H. H.
Right arrow Articles by DeMaria, T. F.

 Previous Article  |  Next Article 

Infection and Immunity, February 2000, p. 921-924, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of the Virulence of a Streptococcus pneumoniae Neuraminidase-Deficient Mutant in Nasopharyngeal Colonization and Development of Otitis Media in the Chinchilla Model

H. H. Tong, L. E. Blue, M. A. James, and T. F. DeMaria*

Division of Otologic Research, College of Medicine and Public Health, The Ohio State University, Columbus, Ohio 43210

Received 8 July 1999/Returned for modification 23 August 1999/Accepted 7 November 1999

Considerable evidence has implicated Streptococcus pneumoniae neuraminidase in the pathogenesis of otitis media (OM); however, its exact role has not been conclusively established. Recently, an S. pneumoniae neuraminidase-deficient mutant, Delta NA1, has been constructed by insertion-duplication mutagenesis of the nanA gene of S. pneumoniae strain D39. The relative ability of Delta NA1 and the D39 parent strain to colonize the nasopharynx and to induce OM subsequent to intranasal inoculation and to survive in the middle ear cleft after direct challenge of the middle ear were evaluated in the chinchilla model. Nasopharyngeal colonization data indicate a significant difference in the ability of the Delta NA1 mutant to colonize as well as to persist in the nasopharynx. The neuraminidase-deficient mutant was eliminated from the nasopharynx 2 weeks earlier than the D39 parent strain. Both the parent and the mutant exhibited similar virulence levels and kinetics during the first week after direct inoculation of the middle ear. The Delta NA1 neuraminidase-deficient mutant, however, was then completely eliminated from the middle ear by day 10 postchallenge, 11 days before the D39 parent strain. Data from this study indicate that products of the nanA gene have an impact on the ability of S. pneumoniae to colonize and persist in the nasopharynx as well as the middle ear.

* Corresponding author. Mailing address: Division of Otologic Research, The Ohio State University, College of Medicine and Public Health, Room 4331 UHC, 456 W. 10th Ave., Columbus, OH 43210. Phone: (614) 293-8103. Fax: (614) 293-5506. E-mail: demaria.2{at}osu.edu.

Infection and Immunity, February 2000, p. 921-924, Vol. 68, No. 2
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chiarezza, M., Lyras, D., Pidot, S. J., Flores-Diaz, M., Awad, M. M., Kennedy, C. L., Cordner, L. M., Phumoonna, T., Poon, R., Hughes, M. L., Emmins, J. J., Alape-Giron, A., Rood, J. I. (2009). The NanI and NanJ Sialidases of Clostridium perfringens Are Not Essential for Virulence. Infect. Immun. 77: 4421-4428 [Abstract] [Full Text]  
  • Parker, D., Soong, G., Planet, P., Brower, J., Ratner, A. J., Prince, A. (2009). The NanA Neuraminidase of Streptococcus pneumoniae Is Involved in Biofilm Formation. Infect. Immun. 77: 3722-3730 [Abstract] [Full Text]  
  • Chiavolini, D., Pozzi, G., Ricci, S. (2008). Animal Models of Streptococcus pneumoniae Disease. Clin. Microbiol. Rev. 21: 666-685 [Abstract] [Full Text]  
  • Mahdi, L. K., Ogunniyi, A. D., LeMessurier, K. S., Paton, J. C. (2008). Pneumococcal Virulence Gene Expression and Host Cytokine Profiles during Pathogenesis of Invasive Disease. Infect. Immun. 76: 646-657 [Abstract] [Full Text]  
  • Abusriwil, H., Stockley, R. A. (2007). The Interaction of Host and Pathogen Factors in Chronic Obstructive Pulmonary Disease Exacerbations and Their Role in Tissue Damage. Proc Am Thorac Soc 4: 611-617 [Abstract] [Full Text]  
  • Nelson, A. L., Roche, A. M., Gould, J. M., Chim, K., Ratner, A. J., Weiser, J. N. (2007). Capsule Enhances Pneumococcal Colonization by Limiting Mucus-Mediated Clearance. Infect. Immun. 75: 83-90 [Abstract] [Full Text]  
  • Marri, P. R., Hao, W., Golding, G. B. (2006). Gene Gain and Gene Loss in Streptococcus: Is It Driven by Habitat?. Mol Biol Evol 23: 2379-2391 [Abstract] [Full Text]  
  • Simell, B., Jaakkola, T., Lahdenkari, M., Briles, D., Hollingshead, S., Kilpi, T. M., Kayhty, H. (2006). Serum Antibodies to Pneumococcal Neuraminidase NanA in Relation to Pneumococcal Carriage and Acute Otitis Media. CVI 13: 1177-1179 [Abstract] [Full Text]  
  • Obert, C., Sublett, J., Kaushal, D., Hinojosa, E., Barton, T., Tuomanen, E. I., Orihuela, C. J. (2006). Identification of a Candidate Streptococcus pneumoniae Core Genome and Regions of Diversity Correlated with Invasive Pneumococcal Disease.. Infect. Immun. 74: 4766-4777 [Abstract] [Full Text]  
  • Manco, S., Hernon, F., Yesilkaya, H., Paton, J. C., Andrew, P. W., Kadioglu, A. (2006). Pneumococcal Neuraminidases A and B Both Have Essential Roles during Infection of the Respiratory Tract and Sepsis. Infect. Immun. 74: 4014-4020 [Abstract] [Full Text]  
  • Tong, H. H., Li, D., Chen, S., Long, J. P., DeMaria, T. F. (2005). Immunization with Recombinant Streptococcus pneumoniae Neuraminidase NanA Protects Chinchillas against Nasopharyngeal Colonization. Infect. Immun. 73: 7775-7778 [Abstract] [Full Text]  
  • Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  
  • Long, J. P., Tong, H. H., DeMaria, T. F. (2004). Immunization with Native or Recombinant Streptococcus pneumoniae Neuraminidase Affords Protection in the Chinchilla Otitis Media Model. Infect. Immun. 72: 4309-4313 [Abstract] [Full Text]  
  • Vimr, E. R., Kalivoda, K. A., Deszo, E. L., Steenbergen, S. M. (2004). Diversity of Microbial Sialic Acid Metabolism. Microbiol. Mol. Biol. Rev. 68: 132-153 [Abstract] [Full Text]  
  • Roche, H., Hakansson, A., Hollingshead, S. K., Briles, D. E. (2003). Regions of PspA/EF3296 Best Able To Elicit Protection against Streptococcus pneumoniae in a Murine Infection Model. Infect. Immun. 71: 1033-1041 [Abstract] [Full Text]  
  • Shakhnovich, E. A., King, S. J., Weiser, J. N. (2002). Neuraminidase Expressed by Streptococcus pneumoniae Desialylates the Lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae: a Paradigm for Interbacterial Competition among Pathogens of the Human Respiratory Tract. Infect. Immun. 70: 7161-7164 [Abstract] [Full Text]  
  • Tong, H. H., Grants, I., Liu, X., DeMaria, T. F. (2002). Comparison of Alteration of Cell Surface Carbohydrates of the Chinchilla Tubotympanum and Colonial Opacity Phenotype of Streptococcus pneumoniae during Experimental Pneumococcal Otitis Media with or without an Antecedent Influenza A Virus Infection. Infect. Immun. 70: 4292-4301 [Abstract] [Full Text]  
  • Jost, B. H., Songer, J. G., Billington, S. J. (2002). Identification of a Second Arcanobacterium pyogenes Neuraminidase and Involvement of Neuraminidase Activity in Host Cell Adhesion. Infect. Immun. 70: 1106-1112 [Abstract] [Full Text]  
  • Jost, B. H., Songer, J. G., Billington, S. J. (2001). Cloning, Expression, and Characterization of a Neuraminidase Gene from Arcanobacterium pyogenes. Infect. Immun. 69: 4430-4437 [Abstract] [Full Text]  
  • Magee, A. D., Yother, J. (2001). Requirement for Capsule in Colonization by Streptococcus pneumoniae. Infect. Immun. 69: 3755-3761 [Abstract] [Full Text]  
  • Mizan, S., Henk, A., Stallings, A., Maier, M., Lee, M. D. (2000). Cloning and Characterization of Sialidases with 2-6' and 2-3' Sialyl Lactose Specificity from Pasteurella multocida. J. Bacteriol. 182: 6874-6883 [Abstract] [Full Text]  
  • Tong, H. H., Blue, L. E., James, M. A., Chen, Y. P., DeMaria, T. F. (2000). Evaluation of Phase Variation of Nontypeable Haemophilus influenzae Lipooligosaccharide during Nasopharyngeal Colonization and Development of Otitis Media in the Chinchilla Model. Infect. Immun. 68: 4593-4597 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»