This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Anguita, J.
Right arrow Articles by Fikrig, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Anguita, J.
Right arrow Articles by Fikrig, E.

 Previous Article  |  Next Article 

Infection and Immunity, March 2000, p. 1222-1230, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Borrelia burgdorferi Gene Expression In Vivo and Spirochete Pathogenicity

Juan Anguita,1 Swapna Samanta,1 Beatriz Revilla,1 Kyoungho Suk,1,dagger Subrata Das,1 Stephen W. Barthold,2 and Erol Fikrig1,*

Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, New Haven, Connecticut 06520,1 and Center for Comparative Medicine, Schools of Medicine and Veterinary Medicine, University of California at Davis, Davis, California 956162

Received 13 October 1999/Returned for modification 19 November 1999/Accepted 30 November 1999

Borrelia burgdorferi spirochetes that do not cause arthritis or carditis were developed and used to investigate Lyme disease pathogenesis. A clonal isolate of B. burgdorferi N40 (cN40), which induces disease in C3H/HeN (C3H) mice, was repeatedly passaged in vitro to generate nonpathogenic spirochetes. The passage 75 isolate (N40-75) was infectious for C3H mice but did not cause arthritis or carditis, and spirochetes were at low levels or absent in the joints or hearts, respectively. N40-75 could, however, cause disease in severe combined immunodeficient (SCID) mice, suggesting that the response in immunocompetent mice prevented effective spirochete dissemination and the subsequent development of arthritis and carditis. Administration of immune sera at 4 days after spirochete challenge aborted N40-75, but not cN40, infection in SCID mice. A B. burgdorferi genomic expression library was differentially probed with sera from cN40- and N40-75-infected mice, to identify genes that may not be effectively expressed by N40-75 in vivo. N40-75 was defective in the up-regulation of several genes that are preferentially expressed during mammalian infection, including dbpAB, bba64, and genes that map to the cp32 family of plasmids. These data suggest that adaptation and gene expression may be required for B. burgdorferi to effectively colonize the host, evade humoral responses, and cause disease.

* Corresponding author. Mailing address: 608 Laboratory of Clinical Investigation, Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520-8031. Phone: (203) 785-2453. Fax: (203) 785-7053. E-mail: ef6{at}emailmed.yale.edu.

dagger Present address: Samsung Biomedical Research Institute, Clinical Research Center, Seoul, South Korea 135-230.

Infection and Immunity, March 2000, p. 1222-1230, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sanjuan, E., Esteve-Gassent, M. D., Maruskova, M., Seshu, J. (2009). Overexpression of CsrA (BB0184) Alters the Morphology and Antigen Profiles of Borrelia burgdorferi. Infect. Immun. 77: 5149-5162 [Abstract] [Full Text]  
  • Maruskova, M., Seshu, J. (2008). Deletion of BBA64, BBA65, and BBA66 Loci Does Not Alter the Infectivity of Borrelia burgdorferi in the Murine Model of Lyme Disease. Infect. Immun. 76: 5274-5284 [Abstract] [Full Text]  
  • Hughes, J. L., Nolder, C. L., Nowalk, A. J., Clifton, D. R., Howison, R. R., Schmit, V. L., Gilmore, R. D. Jr., Carroll, J. A. (2008). Borrelia burgdorferi Surface-Localized Proteins Expressed during Persistent Murine Infection Are Conserved among Diverse Borrelia spp.. Infect. Immun. 76: 2498-2511 [Abstract] [Full Text]  
  • Gautam, A., Hathaway, M., McClain, N., Ramesh, G., Ramamoorthy, R. (2008). Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter. Microbiology 154: 275-285 [Abstract] [Full Text]  
  • Maruskova, M., Esteve-Gassent, M. D., Sexton, V. L., Seshu, J. (2008). Role of the BBA64 Locus of Borrelia burgdorferi in Early Stages of Infectivity in a Murine Model of Lyme Disease. Infect. Immun. 76: 391-402 [Abstract] [Full Text]  
  • Gilmore, R. D. Jr., Howison, R. R., Schmit, V. L., Nowalk, A. J., Clifton, D. R., Nolder, C., Hughes, J. L., Carroll, J. A. (2007). Temporal Expression Analysis of the Borrelia burgdorferi Paralogous Gene Family 54 Genes BBA64, BBA65, and BBA66 during Persistent Infection in Mice. Infect. Immun. 75: 2753-2764 [Abstract] [Full Text]  
  • Blevins, J. S., Revel, A. T., Smith, A. H., Bachlani, G. N., Norgard, M. V. (2007). Adaptation of a Luciferase Gene Reporter and lac Expression System to Borrelia burgdorferi. Appl. Environ. Microbiol. 73: 1501-1513 [Abstract] [Full Text]  
  • Fisher, M. A., Grimm, D., Henion, A. K., Elias, A. F., Stewart, P. E., Rosa, P. A., Gherardini, F. C. (2005). From the Cover: Borrelia burgdorferi {sigma}54 is required for mammalian infection and vector transmission but not for tick colonization. Proc. Natl. Acad. Sci. USA 102: 5162-5167 [Abstract] [Full Text]  
  • Caimano, M. J., Eggers, C. H., Hazlett, K. R. O., Radolf, J. D. (2004). RpoS Is Not Central to the General Stress Response in Borrelia burgdorferi but Does Control Expression of One or More Essential Virulence Determinants. Infect. Immun. 72: 6433-6445 [Abstract] [Full Text]  
  • Wang, G., Iyer, R., Bittker, S., Cooper, D., Small, J., Wormser, G. P., Schwartz, I. (2004). Variations in Barbour-Stoenner-Kelly Culture Medium Modulate Infectivity and Pathogenicity of Borrelia burgdorferi Clinical Isolates. Infect. Immun. 72: 6702-6706 [Abstract] [Full Text]  
  • Eggers, C. H., Caimano, M. J., Radolf, J. D. (2004). Analysis of Promoter Elements Involved in the Transcriptional Initiation of RpoS-Dependent Borrelia burgdorferi Genes. J. Bacteriol. 186: 7390-7402 [Abstract] [Full Text]  
  • Blevins, J. S., Revel, A. T., Caimano, M. J., Yang, X. F., Richardson, J. A., Hagman, K. E., Norgard, M. V. (2004). The luxS Gene Is Not Required for Borrelia burgdorferi Tick Colonization, Transmission to a Mammalian Host, or Induction of Disease. Infect. Immun. 72: 4864-4867 [Abstract] [Full Text]  
  • Babb, K., McAlister, J. D., Miller, J. C., Stevenson, B. (2004). Molecular Characterization of Borrelia burgdorferi erp Promoter/Operator Elements. J. Bacteriol. 186: 2745-2756 [Abstract] [Full Text]  
  • Willett, T. A., Meyer, A. L., Brown, E. L., Huber, B. T. (2004). An effective second-generation outer surface protein A-derived Lyme vaccine that eliminates a potentially autoreactive T cell epitope. Proc. Natl. Acad. Sci. USA 101: 1303-1308 [Abstract] [Full Text]  
  • Iyer, R., Kalu, O., Purser, J., Norris, S., Stevenson, B., Schwartz, I. (2003). Linear and Circular Plasmid Content in Borrelia burgdorferi Clinical Isolates. Infect. Immun. 71: 3699-3706 [Abstract] [Full Text]  
  • Crother, T. R., Champion, C. I., Wu, X.-Y., Blanco, D. R., Miller, J. N., Lovett, M. A. (2003). Antigenic Composition of Borrelia burgdorferi during Infection of SCID Mice. Infect. Immun. 71: 3419-3428 [Abstract] [Full Text]  
  • Pausa, M., Pellis, V., Cinco, M., Giulianini, P. G., Presani, G., Perticarari, S., Murgia, R., Tedesco, F. (2003). Serum-Resistant Strains of Borrelia burgdorferi Evade Complement-Mediated Killing by Expressing a CD59-Like Complement Inhibitory Molecule. J. Immunol. 170: 3214-3222 [Abstract] [Full Text]  
  • Roberts, D. M., Caimano, M., McDowell, J., Theisen, M., Holm, A., Orff, E., Nelson, D., Wikel, S., Radolf, J., Marconi, R. T. (2002). Environmental Regulation and Differential Production of Members of the Bdr Protein Family of Borrelia burgdorferi. Infect. Immun. 70: 7033-7041 [Abstract] [Full Text]  
  • Pachner, A. R., Dail, D., Li, L., Gurey, L., Feng, S., Hodzic, E., Barthold, S. (2002). Humoral Immune Response Associated with Lyme Borreliosis in Nonhuman Primates: Analysis by Immunoblotting and Enzyme-Linked Immunosorbent Assay with Sonicates or Recombinant Proteins. CVI 9: 1348-1355 [Abstract] [Full Text]  
  • Sellek, R. E., Escudero, R., Gil, H., Rodriguez, I., Chaparro, E., Perez-Pastrana, E., Vivo, A., Anda, P. (2002). In Vitro Culture of Borrelia garinii Results in Loss of Flagella and Decreased Invasiveness. Infect. Immun. 70: 4851-4858 [Abstract] [Full Text]  
  • El-Hage, N., Stevenson, B. (2002). Simultaneous Coexpression of Borrelia burgdorferi Erp Proteins Occurs through a Specific, erp Locus-Directed Regulatory Mechanism. J. Bacteriol. 184: 4536-4543 [Abstract] [Full Text]  
  • Stevenson, B., Babb, K. (2002). LuxS-Mediated Quorum Sensing in Borrelia burgdorferi, the Lyme Disease Spirochete. Infect. Immun. 70: 4099-4105 [Abstract] [Full Text]  
  • Anguita, J., Barthold, S. W., Persinski, R., Hedrick, M. N., Huy, C. A., Davis, R. J., Flavell, R. A., Fikrig, E. (2002). Murine Lyme Arthritis Development Mediated by p38 Mitogen-Activated Protein Kinase Activity. J. Immunol. 168: 6352-6357 [Abstract] [Full Text]  
  • Anguita, J., Thomas, V., Samanta, S., Persinski, R., Hernanz, C., Barthold, S. W., Fikrig, E. (2001). Borrelia burgdorferi-Induced Inflammation Facilitates Spirochete Adaptation and Variable Major Protein-Like Sequence Locus Recombination. J. Immunol. 167: 3383-3390 [Abstract] [Full Text]  
  • Hefty, P. S., Jolliff, S. E., Caimano, M. J., Wikel, S. K., Radolf, J. D., Akins, D. R. (2001). Regulation of OspE-Related, OspF-Related, and Elp Lipoproteins of Borrelia burgdorferi Strain 297 by Mammalian Host-Specific Signals. Infect. Immun. 69: 3618-3627 [Abstract] [Full Text]  
  • Babb, K., El-Hage, N., Miller, J. C., Carroll, J. A., Stevenson, B. (2001). Distinct Regulatory Pathways Control Expression of Borrelia burgdorferi Infection-Associated OspC and Erp Surface Proteins. Infect. Immun. 69: 4146-4153 [Abstract] [Full Text]  
  • Thomas, V., Anguita, J., Barthold, S. W., Fikrig, E. (2001). Coinfection with Borrelia burgdorferi and the Agent of Human Granulocytic Ehrlichiosis Alters Murine Immune Responses, Pathogen Burden, and Severity of Lyme Arthritis. Infect. Immun. 69: 3359-3371 [Abstract] [Full Text]  
  • Thomas, V., Anguita, J., Samanta, S., Rosa, P. A., Stewart, P., Barthold, S. W., Fikrig, E. (2001). Dissociation of Infectivity and Pathogenicity in Borrelia burgdorferi. Infect. Immun. 69: 3507-3509 [Abstract] [Full Text]  
  • El-Hage, N., Babb, K., Carroll, J. A., Lindstrom, N., Fischer, E. R., Miller, J. C., Gilmore, R. D. Jr, Mbow, M. L., Stevenson, B. (2001). Surface exposure and protease insensitivity of Borrelia burgdorferi Erp (OspEF-related) lipoproteins. Microbiology 147: 821-830 [Abstract] [Full Text]  
  • Labandeira-Rey, M., Skare, J. T. (2001). Decreased Infectivity in Borrelia burgdorferi Strain B31 Is Associated with Loss of Linear Plasmid 25 or 28-1. Infect. Immun. 69: 446-455 [Abstract] [Full Text]  
  • Carroll, J. A., Cordova, R. M., Garon, C. F. (2000). Identification of 11 pH-Regulated Genes in Borrelia burgdorferi Localizing to Linear Plasmids. Infect. Immun. 68: 6677-6684 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»