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Infection and Immunity, March 2000, p. 1271-1275, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Iron Acquisition from Pseudomonas aeruginosa Siderophores by Human Phagocytes: an Additional Mechanism of Host Defense through Iron Sequestration?

Bradley E. Britigan,1,2,* George T. Rasmussen,1,2 Oyebode Olakanmi,1,2 and Charles D. Cox3

Medical and Research Service, Veterans Administration Medical Center, Iowa City, Iowa 52246,1 and Departments of Internal Medicine2 and Microbiology,3 The University of Iowa College of Medicine, Iowa City, Iowa 52242

Received 15 September 1999/Returned for modification 8 November 1999/Accepted 6 December 1999

Chelation of iron to iron-binding proteins is a strategy of host defense. Some pathogens counter this via the secretion of low-molecular-weight iron-chelating agents (siderophores). Human phagocytes possess a high-capacity mechanism for iron acquisition from low-molecular-weight iron chelates. Efficient acquisition and sequestration of iron bound to bacterial siderophores by host phagocytes could provide a secondary mechanism to limit microbial access to iron. In the present work we report that human neutrophils, macrophages, and myeloid cell lines can acquire iron from the two Pseudomonas aeruginosa siderophores. Analogous to iron acquisition from other low-molecular-weight chelates, iron acquisition from the siderophores is ATP independent, induced by multivalent cationic metals, and unaffected by inhibitors of endocytosis and pinocytosis. In vivo, this process could serve as an additional mechanism of host defense to limit iron availability to invading siderophore-producing microbes.

* Corresponding author. Mailing address: Department of Medicine, The University of Iowa, 200 Hawkins Dr., SW54 GH, Iowa City, IA 52242. Phone: (319) 356-3674. Fax: (319) 356-4600. E-mail: bradley-britigan{at}uiowa.edu.

Infection and Immunity, March 2000, p. 1271-1275, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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