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Infection and Immunity, March 2000, p. 1480-1484, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Influence of the Bcg Locus on Natural Resistance to Primary Infection with the Facultative Intracellular Bacterium Francisella tularensis in Mice

Hana Kovárová,1,* Lenka Hernychová,1 Marián Hajdúch,2 M. Sírová,3 and Ales Macela1

Institute for Immunology and Radiobiology, Purkyne Military Medical Academy, 500 01 Hradec Králové,1 Department of Pediatrics, Faculty of Medicine, Palacky University and Faculty Hospital, 775 15 Olomouc,2 and Institute of Microbiology, Academy of Science of the Czech Republic, 142 20 Prague,3 Czech Republic

Received 3 September 1999/Returned for modification 26 October 1999/Accepted 10 December 1999

The implication of the Bcg locus in the control of natural resistance to infection with a live vaccine strain (LVS) of the intracellular pathogen Francisella tularensis was studied. Analysis of phenotypic expression of natural resistance and susceptibility was performed using mouse strains congenic at the Bcg locus. Comparison of the kinetics of bacterial colonization of spleen showed that B10.A.Bcg(r) mice were extremely susceptible during early phases of primary sublethal infection, while their congenic C57BL/10N [Bcg(s)] counterparts could be classified as resistant to F. tularensis LVS infection according to the 2-log-lower bacterial CFU within the tissue as long as 5 days after infection. Different phenotypes of Bcg congenic mice were associated with differential expression of the cytokines tumor necrosis factor alpha, interleukin-10, and gamma interferon and production of reactive oxygen intermediates. These results strongly suggest that the Bcg locus, which is close or identical to the Nramp1 gene, controls natural resistance to infection by F. tularensis and that its effect is the opposite of that observed for other Bcg-controlled pathogens.

* Corresponding author. Mailing address: Institute for Immunology and Radiobiology, Trebesská Str. 1575, 500 01 Hradec Králové, Czech Republic. Phone: (420 49) 5210833. Fax: (420 49) 5513018. E-mail: kovarova{at}pmfhk.cz.

Infection and Immunity, March 2000, p. 1480-1484, Vol. 68, No. 3
0019-9567/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sullivan, J. T., Jeffery, E. F., Shannon, J. D., Ramakrishnan, G. (2006). Characterization of the Siderophore of Francisella tularensis and Role of fslA in Siderophore Production. J. Bacteriol. 188: 3785-3795 [Abstract] [Full Text]  
  • Guilloteau, L. A., Dornand, J., Gross, A., Olivier, M., Cortade, F., Vern, Y. L., Kerboeuf, D. (2003). Nramp1 Is Not a Major Determinant in the Control of Brucella melitensis Infection in Mice. Infect. Immun. 71: 621-628 [Abstract] [Full Text]  
  • Ellis, J., Oyston, P. C. F., Green, M., Titball, R. W. (2002). Tularemia. Clin. Microbiol. Rev. 15: 631-646 [Abstract] [Full Text]  


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